Comparison of the acute response to meals enriched with cis- or trans-fatty acids on glucose and lipids in overweight individuals with differing FABP2 genotypes

• Published in: Metabolism, clinical and experimental clinical and experimental, 2005-12, Vol.54 (12), p.1652-1658
• Main Authors: Lefevre, Michael, Lovejoy, Jennifer C., Smith, Steven R., DeLany, James P., Champagne, Catherine, Most, Marlene M., Denkins, Yvonne, de Jonge, Lilian, Rood, Jennifer, Bray, George A.
• Format: Article
• Language: English
• Subjects: Adult; Biological and medical sciences; Blood Glucose - analysis; C-Peptide - analysis; Dietary Fats - administration & dosage; Fatty Acid-Binding Proteins - genetics; Fatty Acids, Nonesterified - blood; Female; Genotype; Humans; Insulin - blood; Lipids - blood; Male; Medical sciences; Metabolic diseases; Middle Aged; Obesity; Obesity - metabolism; Postprandial Period; Triglycerides - blood
• ISSN: 0026-0495; 1532-8600
• DOI: 10.1016/j.metabol.2005.06.015

Trans-fatty acids have been implicated as a risk factor for cardiovascular disease and diabetes. In addition, a polymorphism at codon 54 (Ala54Thr) in the fatty acid–binding protein 2 ( FABP2) gene has been suggested to modify an interaction between dietary fat and insulin sensitivity. We examined the postprandial metabolic profiles after meals enriched with C18:1 trans- relative to a similar meal with C18:1 cis-fatty acid in individuals who were either FABP2 Ala54 homozygotes or Thr54 carriers. Moderately overweight men and women ate 2 breakfast test meals, separated by 1 week, each providing 40% of their daily energy requirement and containing 50% of energy as fat. In one meal, 10% of energy was from C18:1 trans, and in the other meal, the C18:1 trans was replaced with C18:1 cis. Metabolic parameters were assessed during an 8-hour period. Insulin and C-peptide levels increased more after the C18:1 trans meal, and this was associated with a greater fall in free fatty acids. Postprandial glucose levels and oxidation of fatty acids and carbohydrate were not different between the 2 test meals. The Thr54 allele for FABP2 increased the rise in postprandial glucose but not triacylglycerols. Fractional triacylglycerol synthetic rates were higher after consumption of the C18:1 trans meal relative to the C18:1 cis meal only in Thr54 carriers. These data show that a single meal enriched with C18:1 trans-fatty acids can significantly increase insulin resistance, and that in the presence of the FABP2 Thr54 allele, may contribute to increased partitioning of glucose to triacylglycerols and insulin resistance.