Multiple Label-Free Detection of Antigen−Antibody Reaction Using Localized Surface Plasmon Resonance-Based Core−Shell Structured Nanoparticle Layer Nanochip

In this research, a localized surface plasmon resonance (LSPR)-based bioanalysis method for developing multiarray optical nanochip suitable for screening bimolecular interactions is described. LSPR-based label-free monitoring enables to solve the problems of conventional methods that require large s...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2006-09, Vol.78 (18), p.6465-6475
Main Authors: Endo, Tatsuro, Kerman, Kagan, Nagatani, Naoki, Hiepa, Ha Minh, Kim, Do-Kyun, Yonezawa, Yuji, Nakano, Koichi, Tamiya, Eiichi
Format: Article
Language:English
Subjects:
Analytical chemistry
Antigen-Antibody Reactions
Antigens
Antigens - analysis
C-Reactive Protein - chemistry
Chemistry
Exact sciences and technology
Fibrinogen - chemistry
Immunoglobulins
Immunoglobulins - chemistry
Medical diagnosis
Miscellaneous
Nanoparticles
Nanoparticles - chemistry
Proteins
Proteomics - instrumentation
Proteomics - methods
Surface Plasmon Resonance - methods
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Summary:In this research, a localized surface plasmon resonance (LSPR)-based bioanalysis method for developing multiarray optical nanochip suitable for screening bimolecular interactions is described. LSPR-based label-free monitoring enables to solve the problems of conventional methods that require large sample volumes and time-consuming labeling procedures. We developed a multiarray LSPR-based nanochip for the label-free detection of proteins. The multiarray format was constructed by a core−shell-structured nanoparticle layer, which provided 300 nanospots on the sensing surface. Antibodies were immobilized onto the nanospots using their interaction with Protein A. The concentrations of antigens were determined from the peak absorption intensity of the LSPR spectra. We demonstrated the capability of the array measurement using immunoglobulins (IgA, IgD, IgG, IgM), C-reactive protein, and fibrinogen. The detection limit of our label-free method was 100 pg/mL. Our nanochip is readily transferable to monitor the interactions of other biomolecules, such as whole cells or receptors, with a massively parallel detection capability in a highly miniaturized package. We anticipate that the direct label-free optical immunoassay of proteins reported here will revolutionize clinical diagnosis and accelerate the development of hand-held and user-friendly point-of-care devices.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac0608321