Serotonin and 5-hydroxy-indole-acetic acid contents in dorsal raphe and suprachiasmatic nuclei in normal, malnourished and rehabilitated rats under 24 h of sleep deprivation

• Published in: Brain research 2006-09, Vol.1110 (1), p.95-101
• Main Authors: Alfaro-Rodríguez, A., González-Piña, R., González-Maciel, A., Arch-Tirado, E.
• Format: Article
• Language: English
• Subjects: 5-Hydroxy-indole-acetic acid; Animals; Behavior, Animal; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Disorders of higher nervous function. Focal brain diseases. Central vestibular syndrome and deafness. Brain stem syndromes; Dorsal raphe nuclei; Fundamental and applied biological sciences. Psychology; Hydroxyindoleacetic Acid - metabolism; Male; Malnutrition; Malnutrition - metabolism; Malnutrition - pathology; Malnutrition - rehabilitation; Medical sciences; Nervous system (semeiology, syndromes); Neurology; Raphe Nuclei - metabolism; Rats; Rats, Sprague-Dawley; Serotonin; Serotonin - metabolism; Sleep deprivation; Sleep Deprivation - metabolism; Sleep. Vigilance; Suprachiasmatic nuclei; Suprachiasmatic Nucleus - metabolism; Vertebrates: nervous system and sense organs
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2006.06.069

It has been discussed that serotonin (5-HT) could be involved in the effects of sleep deprivation (SD) and/or malnutrition (M) on the sleep–wake cycle. The aim of this work was to study the effects of the M, SD and its interaction on 5-HT and 5-hydroxy-indole-acetic acid (5-HIAA) contents in the dorsal raphe (DR) and the suprachiasmatic nuclei (SCN), two sleep–wake cycle regulators. Forty-eight puppets rats were obtained from mothers fed with low or normal casein diet. They were allocated in 3 groups ( n = 16 each): prenatal/postnatal casein malnutrition (6/6%), prenatal casein malnutrition/nutritional casein rehabilitation (6/25%) and prenatal/postnatal casein well-nourished state (25/25%). When rats were 60 days old, 24 animals were exposed to sleep deprivation by means of forced locomotion during 24 h. The remaining 24 were kept under normal conditions of sleep–wake cycle. Then, all animals were sacrificed by decapitation. DR and SCN were dissected and processed to determine the 5-HT and 5-HIAA contents by means of HPLC. It was observed that 6/6% rats showed a 5-HT increase (DR p < 0.011; SCN p < 0.019) as well as in SD (DR p < 0.0008; SCN p < 0.0009) with respect to 25/25% rats. No differences were found in 6/25% rats. Therefore, 5-HIAA decreased significantly in both nuclei in all the groups, notably in M + SD animals (DR p < 0.001; SCN p < 0.001). We conclude that the sleep–wake cycle disruptions produced by chronic M and SD are mediated in part by a synergistic effect on 5-HT in the DR-SCN pathway, perhaps due to a delay in the development of such brain structures.