New application of air-drying techniques for studying Ephemeroptera and Plecoptera eggs by scanning electron microscopy

Hexamethyldisilizane (HMDS) and tetramethylsilane are organic compounds that are volatile at ambient temperature and which can therefore be used for air‐drying biological samples for SEM studies. The techniques using these compounds provide results that are comparable with those obtained by critical...

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Bibliographic Details
Published in:Microscopy research and technique 2005-12, Vol.68 (5), p.264-271
Main Authors: Ubero-Pascal, Nicolás, Fortuño, José Manuel, de Los Angeles Puig, María
Format: Article
Language:English
Subjects:
air-drying techniques
Animals
critical point drying
Desiccation - methods
egg morphology
Ephemeroptera
Female
hexamethyldisilizane
Histocytological Preparation Techniques - methods
Insecta - growth & development
Insecta - ultrastructure
Microscopy, Electron, Scanning - methods
Organosilicon Compounds
Ovum - ultrastructure
Plecoptera
SEM
Silanes
tetramethylsilane
Trimethylsilyl Compounds
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Summary:Hexamethyldisilizane (HMDS) and tetramethylsilane are organic compounds that are volatile at ambient temperature and which can therefore be used for air‐drying biological samples for SEM studies. The techniques using these compounds provide results that are comparable with those obtained by critical point drying, but which involve a very simple process that saves time and money. Both techniques were applied to SEM studies of Ephemeroptera and Plecoptera eggs in order to assess their suitability as alternative methods to critical point drying for these kinds of biological material. The results show no morphological differences between eggs HMDS air‐dried and critical point‐dried. Microsc. Res. Tech. 68:264–271, 2005. © 2005 Wiley‐Liss, Inc.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.20248