DNA markers for identifying biotypes B and Q of Bemisia tabaci (Hemiptera: Aleyrodidae) and studying population dynamics

• Published in: Bulletin of entomological research 2005-12, Vol.95 (6), p.605-613
• Main Authors: Khasdan, V., Levin, I., Rosner, A., Morin, S., Kontsedalov, S., Maslenin, L., Horowitz, A.R.
• Format: Article
• Language: English
• Subjects: Aleyrodidae; Animal and plant ecology; Animal, plant and microbial ecology; Animals; Bemisia argentifolii; Bemisia tabaci; Biological and medical sciences; biotype Q; Biotypes; cleaved amplified polymorphic sequences (CAPS); cytochrome-c oxidase; Demecology; Deoxyribonucleic acid; DNA; DNA markers; Entomology; Fundamental and applied biological sciences. Psychology; genes; Genes, Insect; Genetic diversity; Genetic Markers; genetic polymorphism; genetic techniques and protocols; genetic variation; Genetics, Population; Hemiptera; Hemiptera - classification; Hemiptera - genetics; Insecticides; Israel; mitochondrial DNA; pest identification; Phytopathology. Animal pests. Plant and forest protection; Polymorphism; Population Dynamics; population genetics; Protozoa. Invertebrata; Protozoa. Invertebrates; Random Amplified Polymorphic DNA Technique; Records, symptoms, damages, economic importance, population surveys; Sequence Analysis, DNA; sequence characterized amplified regions (SCAR); sodium channel gene; whitefly
• ISSN: 0007-4853; 1475-2670
• DOI: 10.1079/BER2005390

The two most widespread biotypes of Bemisia tabaci (Gennadius) in southern Europe and the Middle East are referred to as the B and Q-type, which are morphologically indistinguishable. In this study various DNA markers have been developed, applied and compared for studying genetic diversity and distribution of the two biotypes. For developing sequence characterized amplified regions (SCAR) and cleaved amplified polymorphic sequences (CAPS) techniques, single random amplified polymorphic DNA (RAPD) fragments of B and Q biotypes, respectively, were used. The CAPS were investigated on the basis of nuclear sodium channel and the mitochondrial cytochrome oxidase I genes (mtCOI) sequences. In general, complete agreement was found between the different markers used. Analysis of field samples collected in Israel for several years, using these markers, indicated that the percentage of the Q biotype tends to increase in field populations as time progresses. This may be attributed to the resistance of the Q biotype to neonicotinoids and pyriproxyfen and the susceptibility of the B biotype to these insecticides.