Ischemia-induced modifications in hippocampal CA1 stratum radiatum excitatory synapses

• Published in: Hippocampus 2006-01, Vol.16 (10), p.814-825
• Main Authors: Kovalenko, Tatiana, Osadchenko, Irina, Nikonenko, Alexander, Lushnikova, Irina, Voronin, Kirill, Nikonenko, Irina, Muller, Dominique, Skibo, Galina
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn; Cell Death - physiology; dendritic spines; Disease Models, Animal; electron microscopy; Excitatory Postsynaptic Potentials - physiology; Hippocampus - blood supply; Hippocampus - pathology; Hippocampus - physiopathology; Hypoxia-Ischemia, Brain - pathology; Hypoxia-Ischemia, Brain - physiopathology; Microscopy, Electron, Transmission; morphometry; Nerve Degeneration - etiology; Nerve Degeneration - pathology; Nerve Degeneration - physiopathology; Nerve Regeneration - physiology; Neural Pathways - blood supply; Neural Pathways - pathology; Neural Pathways - physiopathology; Neuronal Plasticity - physiology; Organ Culture Techniques; Presynaptic Terminals - pathology; Pyramidal Cells - pathology; Rats; Synapses - pathology; Synaptic Membranes - pathology; synaptic plasticity; Synaptic Transmission - physiology; synaptic vesicles; Synaptic Vesicles - pathology
• ISSN: 1050-9631; 1098-1063
• DOI: 10.1002/hipo.20211

Relatively mild ischemic episode can initiate a chain of events resulting in delayed cell death and significant lesions in the affected brain regions. We studied early synaptic modifications after brief ischemia modeled in rats by transient vessels' occlusion in vivo or oxygen–glucose deprivation in vitro and resulting in delayed death of hippocampal CA1 pyramidal cells. Electron microscopic analysis of excitatory spine synapses in CA1 stratum radiatum revealed a rapid increase of the postsynaptic density (PSD) thickness and length, as well as formation of concave synapses with perforated PSD during the first 24 h after ischemic episode, followed at the long term by degeneration of 80% of synaptic contacts. In presynaptic terminals, ischemia induced a depletion of synaptic vesicles and changes in their spatial arrangement: they became more distant from active zones and had larger intervesicle spacing compared to controls. These rapid structural synaptic changes could be implicated in the mechanisms of cell death or adaptive plasticity. Comparison of the in vivo and in vitro model systems used in the study demonstrated a general similarity of these early morphological changes, confirming the validity of the in vitro model for studying synaptic structural plasticity. © 2006 Wiley‐Liss, Inc.