Hypoxia can contribute to the induction of the Epstein-Barr virus (EBV) lytic cycle

Like other herpes viruses, latent Epstein-Barr virus (EBV) infection can be reactivated to lytic replication. Reactivation can be achieved by treatment with various reagents, including tetradecanoyl phorbol acetate (TPA) and Ca 2+ ionophores. Relatively little is known about the physiological factor...

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Bibliographic Details
Published in:Journal of clinical virology 2006-10, Vol.37 (2), p.98-103
Main Authors: Jiang, Ju-Hong, Wang, Na, Li, Ang, Liao, Wen-Ting, Pan, Zhi-Gang, Mai, Shi-Juan, Li, Da-Jiang, Zeng, Mu-Sheng, Wen, Jian-ming, Zeng, Yi-Xin
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Callithrix
Cell Cycle
Cell Hypoxia
DNA-Binding Proteins - genetics
Epstein-Barr virus
Fundamental and applied biological sciences. Psychology
Gene Dosage
Genes, Immediate-Early
Herpesvirus 4, Human - physiology
Human viral diseases
Hypoxia
Hypoxia-Inducible Factor 1, alpha Subunit - genetics
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
Nasopharyngeal carcinoma
Promoter Regions, Genetic
Reactivation
Trans-Activators - genetics
Tumors
Viral diseases
Viral Proteins - genetics
Virology
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Summary:Like other herpes viruses, latent Epstein-Barr virus (EBV) infection can be reactivated to lytic replication. Reactivation can be achieved by treatment with various reagents, including tetradecanoyl phorbol acetate (TPA) and Ca 2+ ionophores. Relatively little is known about the physiological factors related to reactivation of EBV. Previous studies have demonstrated that G 0/G 1 cell cycle arrest is associated with EBV activation, and that hypoxic conditions can induce cell cycle arrest. In the present study we investigated the effect of hypoxia on reactivation of EBV. Hypoxic culture conditions were established and the expression of Zta protein and the number of EBV DNA copies were measured in B95-8 cells maintained under these conditions. Hypoxia treatment not only increased the expression of the EBV immediate-early protein Zta (which mediates the switch between the latent and lytic form of infection), but also increased the number of EBV DNA copies in B95-8 cells. EBV in latent infection can be activated to lytic infection by hypoxia treatment.
ISSN:1386-6532
1873-5967
DOI:10.1016/j.jcv.2006.06.013