Epidemiological studies on Theileriosis and the dynamics of Theileria parva infections in Rwanda

• Published in: Veterinary parasitology 2007-02, Vol.143 (3), p.214-221
• Main Authors: Bazarusanga, T., Vercruysse, J., Marcotty, T., Geysen, D.
• Format: Article
• Language: English
• Subjects: Agro-ecological zones; agroecological zones; Animals; Arachnid Vectors - parasitology; Cattle; Cattle Diseases - epidemiology; disease prevalence; DNA, Protozoan - analysis; dry season; epidemiological studies; Epidemiology; geographical variation; highlands; Ixodidae; lowlands; PCR; Polymerase Chain Reaction - methods; Polymerase Chain Reaction - veterinary; Polymorphism, Restriction Fragment Length; Prevalence; Rwanda; Rwanda - epidemiology; seasonal variation; Seasons; Species Specificity; Theileria parva; Theileria parva - isolation & purification; Theileriasis - epidemiology; theileriosis; Tick Control; tick-borne diseases; Ticks - parasitology; wet season
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2006.09.009

An epidemiological analysis based on three country wide surveys was carried out to determine the prevalence of infections with Theileria spp. in Rwanda. In the 1998 dry season, a total of 264 blood samples were submitted to Theileria spp. characterisation using the 18S species-specific PCR-RFLP assay. The same samples together with 634 samples (317 samples/season) collected during the 2002 dry season and the 2003 wet season were further analysed using the p104 Theileria parva specific PCR. The results from the 18S characterisation showed the presence of four Theileria spp., namely T. parva, T. mutans, T. taurotragi and T. velifera in the field. Half of the animals had multiple Theileria spp. infections. T. parva was the most prevalent and a high correlation (94%) was found between the prevalence results using the 18S and the p104 PCR assays. The prevalence of T. parva infections was stable over time and over season but decreased significantly from the high land to the low land areas. This unexpected trend cannot be explained alone by ecology or the dynamics of the tick population in the different zones, many other components such as breed type, tick control practices and grazing system are likely to play a role. Another important finding was the fact that young animals are infected early in life in all regions except in the high land zone indicating the existence of a particular epidemiological situation in this part of the country.