Immobilized Hydrogels for Screening of Molecular Interactions

Spatially arrayed, high-density microarrays enable the rapid assessment of biological recognition events, and this information is of widespread interest for those working in basic research laboratories as well as in the clinic. Today, one can find DNA, protein, or small molecule arrays. Limitations...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2007-02, Vol.79 (3), p.1064-1066
Main Authors: Dominguez, Melissa M, Wathier, Michel, Grinstaff, Mark W, Schaus, Scott E
Format: Article
Language:English
Subjects:
Analytical chemistry
Binding Sites
Biocompatible Materials
Chemistry
Exact sciences and technology
General, instrumentation
Hydrogels
Molecular biology
Molecular Probe Techniques
Nucleic Acids - metabolism
Organic Chemicals - metabolism
Proteins - metabolism
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Summary:Spatially arrayed, high-density microarrays enable the rapid assessment of biological recognition events, and this information is of widespread interest for those working in basic research laboratories as well as in the clinic. Today, one can find DNA, protein, or small molecule arrays. Limitations with these systems include covalent modification of the target complement to the array substrate, array- and target-dependent setup conditions, multiple steps, and loss of hydration at the surface. To overcome these limitations, we have designed, prepared, and evaluated immobilized hydrogels as general screening chambers for small molecule−protein, protein−protein, and nucleic acid−nucleic acid interactions. This biomaterial-based approach is facile, rapid, requires only one setup protocol, and physically entraps the target complement within the polymer network and thus offers advantages over the conventional chips.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac061709c