The lysine-ketoglutarate reductase–saccharopine dehydrogenase is involved in the osmo-induced synthesis of pipecolic acid in rapeseed leaf tissues

• Published in: Plant physiology and biochemistry 2006-07, Vol.44 (7), p.474-482
• Main Authors: Moulin, M., Deleu, C., Larher, F., Bouchereau, A.
• Format: Article
• Language: English
• Subjects: Acetyl Coenzyme A - metabolism; Biological and medical sciences; Brassica napus; Brassica rapa - physiology; Enzyme Activation; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant; l-Lysine catabolism; Lysine - metabolism; Metabolism; Nitrogen metabolism; Osmotic Pressure; Osmotic stress; Pipecolic acid; Pipecolic Acids - metabolism; Plant Leaves - physiology; Plant physiology and development; Plant Proteins - physiology; Saccharopine Dehydrogenases - physiology; α-Aminoadipate semialdehyde
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2006.08.005

Higher plant responses to abiotic stresses are associated with physiological and biochemical changes triggering a number of metabolic adjustments. We focused on l-lysine catabolism, and have previously demonstrated that degradation of this amino acid is osmo-regulated at the level of lysine-ketoglutarate reductase (LKR, EC 1.5.1.8) and saccharopine dehydrogenase (SDH, EC 1.5.1.9) in Brassica napus. LKR and SDH activities are enhanced by decreasing osmotic potential and decrease when the upshock osmotic treatment is followed by a downshock osmotic one. Moreover we have shown that the B. napus LKR/SDH gene is up-regulated in osmotically-stressed tissues. The LKR/SDH activity produces α-aminoadipate semialdehyde which could be further converted into α-aminoadipate and acetyl CoA. Alternatively α-aminoadipate could behave as a precursor for pipecolic acid. Pipecolic acid is described as an osmoprotectant in bacteria and is co-accumulated with proline in halophytic plants. We suggest that osmo-induction of the LKR/SDH activity could be partly responsible for pipecolic acid accumulation. This proposal has been assessed in this study through pipecolic acid amounts determination in rape leaf discs subjected to various upshift and downshift osmotic treatments. Changes in pipecolic acid level actually behave as those observed for LKR and SDH activities, since it increases or decreases in rape leaf discs treated under hyper- or hypo-osmotic conditions, respectively. In addition we show that pipecolic acid level is positively correlated with the external osmotic potential as well as with the duration of the applied treatment. On the other hand pipecolic acid level is related to the availability of l-lysine and not to that of d-lysine. Collectively the results obtained demonstrate that lysine catabolism through LKR/SDH activity is involved in osmo-induced synthesis of pipecolic acid.