A proteomics approach for identifying osmotic-stress-related proteins in rice

• Published in: Phytochemistry (Oxford) 2007-02, Vol.68 (4), p.426-437
• Main Authors: Zang, Xin, Komatsu, Setsuko
• Format: Article
• Language: English
• Subjects: abscisic acid; Amino Acid Sequence; Basal part of leaf sheath; Biological and medical sciences; cultivars; Freshwater; Fundamental and applied biological sciences. Psychology; Graminacea; Mannitol; Mannitol - metabolism; Oryza - growth & development; Oryza - physiology; Oryza sativa; Osmotic Pressure; Osmotic stress; Peptide Fragments - chemistry; physiological response; Plant physiology and development; plant proteins; Plant Proteins - chemistry; Plant Proteins - metabolism; plant response; Plant Shoots - growth & development; plant stress; protein content; Proteomics; Rice; two-dimensional gel electrophoresis; Water and solutes. Absorption, translocation and permeability
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2006.11.005

To investigate the mechanisms of how plants respond to osmotic stress, rice protein profiles from mannitol-treated plants, were monitored using a proteomics approach. specific proteins expressed in the basal part of rice leaf sheaths show a coordinated response to cope with osmotic stress. Osmotic stress can endanger the survival of plants. To investigate the mechanisms of how plants respond to osmotic stress, rice protein profiles from mannitol-treated plants, were monitored using a proteomics approach. Two-week-old rice seedlings were treated with 400 mM mannitol for 48 h. After separation of proteins from the basal part of leaf sheaths by two-dimensional polyacrylamide gel electrophoresis, 327 proteins were detected. The levels of 12 proteins increased and the levels of three proteins decreased with increasing concentration or duration, of mannitol treatment. Levels of a heat shock protein and a dnaK-type molecular chaperone were reduced under osmotic, cold, salt and drought stresses, and ABA treatment, whereas a 26S proteasome regulatory subunit was found to be responsive only to osmotic stress. Furthermore, proteins whose accumulation was sensitive to osmotic stress are present in an osmotic-tolerant cultivar. These results indicate that specific proteins expressed in the basal part of rice leaf sheaths show a coordinated response to cope with osmotic stress.