Replication of Flock House Virus in Three Genera of Medically Important Insects

Flock House Virus (family Nodaviridae, genus Alphanodavirus, FHV) was originally isolated from grass grubs Costelytra zealandica (White) (Coleoptera: Scarabaeidae) in New Zealand and belongs to a family of divided genome, plus-sense RNA insect viruses. FHV replicates in insects, a nematode, plants,...

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Bibliographic Details
Published in:Journal of medical entomology 2007-01, Vol.44 (1), p.102-110
Main Authors: Dasgupta, Ranjit, Free, Heather M., Zietlow, Suzanne L., Paskewitz, Susan M., Aksoy, Serap, Shi, Lei, Fuchs, Jeremy, Hu, Changyun, Christensen, Bruce M.
Format: Article
Language:English
Subjects:
Animals
Anopheles gambiae
Anopheles stephensi
Biological and medical sciences
Cell Line
disseminated infection
dose response
Flock House virus
Fundamental and applied biological sciences. Psychology
Glossina morsitans morsitans
host range
insect vectors
Insect Vectors - virology
insect viruses
Insecta
Insecta - virology
Invertebrates
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
mortality
mosquitoes
Nodaviridae - growth & development
reduviid
Rhodnius prolixus
Species Specificity
Survival Analysis
Time Factors
tissue distribution
tsetse
VECTOR/PATHOGEN/HOST INTERACTION, TRANSMISSION
Vectors. Intermediate hosts
virus replication
Virus Replication - physiology
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Description
Summary:Flock House Virus (family Nodaviridae, genus Alphanodavirus, FHV) was originally isolated from grass grubs Costelytra zealandica (White) (Coleoptera: Scarabaeidae) in New Zealand and belongs to a family of divided genome, plus-sense RNA insect viruses. FHV replicates in insects, a nematode, plants, and yeast. We previously reported replication of FHV in four genera of mosquitoes and expression of green fluorescent protein in Aedes aegypti (L.) produced by an FHV-based vector. We report here that FHV multiplies vigorously in vivo in the malaria vectors Anopheles gambiae Giles and An. stephensi Liston and in vitro in a cell line derived from An. gambiae. In addition, FHV multiplies extensively in two other medically important insects, the tsetse fly, Glossina morsitans morsitans Westwood, and the reduviid bug Rhodnius prolixus Stal, extending its host range to four orders of insects (Coleoptera, Lepidoptera, Diptera, and Hemiptera). The virus disseminates in all the major tissues of the insects studied. Anopheles and Glossina show mortality when FHV is injected at a dose above 104 plaque-forming units (pfu) or the virus accumulates to titer above 108 pfu. A lower dose (103 pfu) promotes more extensive virus multiplication and reduces mortality to
ISSN:0022-2585
1938-2928
DOI:10.1603/0022-2585%282007%2944%5B102%3AROFHVI%5D2.0.CO%3B2