A Toll receptor from Chinese shrimp Fenneropenaeus chinensis is responsive to Vibrio anguillarum infection

Toll-like receptors (TLRs) are an evolutionarily ancient family of pattern recognition receptors (PRRs), playing a crucial role in innate immune responses. Here we present a Toll homolog from Chinese shrimp Fenneropenaeus chinensis, designated FcToll. The full-length cDNA of FcToll is 4115 bp includ...

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Bibliographic Details
Published in:Fish & shellfish immunology 2008-05, Vol.24 (5), p.564-574
Main Authors: Yang, Changjian, Zhang, Jiquan, Li, Fuhua, Ma, Hongming, Zhang, Qingli, Jose Priya, T.A., Zhang, Xiaojun, Xiang, Jianhai
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Fenneropenaeus chinensis
Gene Expression Regulation
Gene Order
Innate immunity
Marine
Molecular Sequence Data
Penaeidae - genetics
Penaeidae - immunology
Penaeidae - microbiology
Phylogeny
Real-time PCR
Sequence Alignment
Shrimp
Toll-like receptor
Toll-Like Receptors - chemistry
Toll-Like Receptors - genetics
Toll-Like Receptors - immunology
Vibrio - immunology
Vibrio anguillarum
White spot syndrome virus
WSSV
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Summary:Toll-like receptors (TLRs) are an evolutionarily ancient family of pattern recognition receptors (PRRs), playing a crucial role in innate immune responses. Here we present a Toll homolog from Chinese shrimp Fenneropenaeus chinensis, designated FcToll. The full-length cDNA of FcToll is 4115 bp including a poly A-tail of 16 bp, encoding a putative protein of 931 amino acids. The predicted protein consists of an extracellular domain with a potential signal peptide, 16 leucine-rich repeats (LRR), two LRR-C-terminal (LRR-CT) motifs, and two LRR-N-terminal (LRR-NT) motifs, followed by a transmembrane segment of 23 amino acids, and a cytoplasmic Toll/Interleukin-1R (TIR) domain of 139 residues. Genomic structure of FcToll gene contains five exons and four introns. Phylogenetic analysis revealed that it belongs to insect-type invertebrate Toll family. Transcripts of FcToll gene were constitutively expressed in various tissues, with predominant level in lymphoid organ. Real-time PCR assays demonstrated that expression patterns of FcToll were distinctly modulated after bacterial or viral stimulation, with significant enhancement after 5 h post- Vibrio anguillarum challenge but markedly reduced levels immediately after white spot syndrome virus (WSSV) exposure. These results suggest that FcToll might be involved in innate host defense, especially against the pathogen V. anguillarum.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2007.12.012