Visualisation of the kinetics of macrophage infiltration during experimental autoimmune encephalomyelitis by magnetic resonance imaging

Abstract Macrophages are considered to be the predominant effector cells in multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE). Ultra small particles of iron oxide (USPIO) can be used to detect macrophage infiltrates in the CNS with magnetic resonance imagin...

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Bibliographic Details
Published in:Journal of neuroimmunology 2008-03, Vol.195 (1), p.1-6
Main Authors: Baeten, Kurt, Hendriks, Jerome JA, Hellings, Niels, Theunissen, Evi, Vanderlocht, Joris, Ryck, Leen De, Gelan, Jan, Stinissen, Piet, Adriaensens, Peter
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antigens, CD - metabolism
Antigens, Differentiation, Myelomonocytic - metabolism
Cell Migration Assays, Macrophage
Encephalomyelitis, Autoimmune, Experimental - pathology
Encephalomyelitis, Autoimmune, Experimental - physiopathology
Experimental autoimmune encephalomyelitis
Female
Ferric Compounds - pharmacokinetics
Macrophages - drug effects
Macrophages - physiology
Magnetic Resonance Imaging
Multiple sclerosis
Myelin Basic Protein - adverse effects
Neurology
Rats
Rats, Inbred Lew
Time Factors
Ultra small particles of iron oxide
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Summary:Abstract Macrophages are considered to be the predominant effector cells in multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE). Ultra small particles of iron oxide (USPIO) can be used to detect macrophage infiltrates in the CNS with magnetic resonance imaging (MRI). Here, we investigated whether the kinetics of lesion formation in EAE can be visualised by altering the time point of USPIO injection and the time interval between particle injection and MRI. When USPIO are systemically injected 24 h before MRI, hypo intense regions are detected in different brain regions depending on the disease stage. These regions correspond to sites of macrophage infiltration. A more complete visualisation of sites of inflammation is accomplished by USPIO injection at disease onset and postponing MRI to top of disease. This study demonstrates that the distribution pattern and amount of inflammatory lesions detected with USPIO, depends on timing of USPIO administration and subsequent MRI. These findings are important for a correct application and interpretation of USPIO dependent contrast imaging of CNS inflammation.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2007.11.008