The microbiome of the cloacal openings of the urogenital and anal tracts of the tammar wallaby, Macropus eugenii

1 Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, NSW 2109, Australia 2 CSIRO Entomology, GPO Box 1700, Canberra, ACT 2601, Australia 3 Institute of Dental Research, Westmead Millennium Institute and Westmead Center for Oral Health, Westmead, NSW...

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Published in:Microbiology (Society for General Microbiology) 2008-05, Vol.154 (5), p.1535-1543
Main Authors: Chhour, Kim-Ly, Hinds, Lyn A, Deane, Elizabeth M, Jacques, Nicholas A
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Animals
Bacteriology
Biodiversity
Biological and medical sciences
Cloaca - microbiology
DNA Fingerprinting
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Female
Fundamental and applied biological sciences. Psychology
Gastrointestinal Tract - microbiology
Macropodidae - microbiology
Metagenome
Microbial ecology
Microbiology
Miscellaneous
Molecular Sequence Data
Normal microflora of man and animals. Rumen
Phylogeny
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Urogenital System - microbiology
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Summary:1 Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, NSW 2109, Australia 2 CSIRO Entomology, GPO Box 1700, Canberra, ACT 2601, Australia 3 Institute of Dental Research, Westmead Millennium Institute and Westmead Center for Oral Health, Westmead, NSW 2145, Australia Correspondence Elizabeth M. Deane elizabeth.deane{at}anu.edu.au The bacterial diversity of the openings of the urogenital and anal tracts of the adult female tammar wallaby, Macropus eugenii , was determined in order to ascertain whether the physical proximity of the openings of these tracts within the cloaca affected the two populations of bacteria. Terminal restriction fragment length polymorphism (T-RFLP) analyses of 42 wallabies identified 81 different terminal fragments, indicative of diverse and complex microbiomes at these anatomical locations. Subsequent amplified rDNA restriction analysis (ARDRA) identified 72 phylotypes from the urogenital tract and 50 from the anal tract. Twenty-two of these phylotypes were common to both tracts. Phylogenetic analysis of sequenced 16S rDNA showed that 83 % of the phylotypes were unidentified species based on the premise that any sequence possessing
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.2007/014803-0