Changes in Gingival Crevicular Fluid Levels of Immunoglobulin A Following Therapy: Association With Attachment Loss

• Published in: Journal of periodontology (1970) 1999-10, Vol.70 (10), p.1221-1227
• Main Authors: Grbic, John T., Lamster, Ira B., Fine, James B., Lam, Kei S., Celenti, Romanita S., Herrera‐Abreu, Miriam, Singer, Robert E.
• Format: Article
• Language: English
• Subjects: Adult; Analysis of Variance; Biomarkers - analysis; Clinical Protocols; Dental Scaling; Dentistry; follow‐up studies; Gingival crevicular fluid; Gingival Crevicular Fluid - chemistry; glucuronidase; Glucuronidase - analysis; Humans; IgA; IgG; Immunoglobulin A - analysis; Immunoglobulin G - analysis; Lysosomes - enzymology; periodontal attachment loss; Periodontal Attachment Loss - diagnosis; Periodontal Attachment Loss - therapy; planing; Root Planing; scaling; Time Factors
• ISSN: 0022-3492; 1943-3670
• DOI: 10.1902/jop.1999.70.10.1221

Background: In previous studies, we demonstrated that increased levels of immunoglobulin A (IgA) in gingival crevicular fluid (GCF) may be “protective,” while increased levels of the polymorphonuclear lysosomal enzyme, β‐glucuronidase, in GCF were associated with increased risk of disease activity. In this study, we examined the effect of scaling and root planing (SRP) on the levels of β‐glucuronidase, IgG, and IgA in GCF over a 24‐week period and compared these to clinical attachment loss (CAL). Methods: Twenty‐nine patients with periodontal disease were examined for attachment level, probing depth, plaque, and bleeding on probing at 6 sites per tooth. GCF was collected from the mesial aspect of all teeth excluding third molars and analyzed for β‐glucuronidase, IgG, and IgA. After baseline data were collected, each patient received SRP, and GCF was collected again at 2, 4, 6, 8, 12, and 24 weeks post‐SRP while clinical data were obtained at 4, 8, 12, and 24 weeks. In addition, we analyzed whether the magnitude of the IgA response to SRP would affect the rate of periodontal disease progression by examining GCF IgA levels at 2 time intervals: 2 to 4 weeks post‐SRP and 6 to 12 weeks post‐SRP. Results: Seventeen patients (58.6%) exhibited at least 1 site losing ≥2.5 mm of CAL during the 24‐week study. β‐glucuronidase in GCF was significantly decreased at 2 weeks following SRP and then demonstrated a gradual increase throughout the study period. Levels of IgA in GCF significantly increased following SRP, reaching a peak at 6 weeks and then gradually decreasing throughout the study. Furthermore, we found an inverse relationship between GCF IgA levels at 6 to 12 weeks post‐SRP and the occurrence of CAL. Conclusions: These results support the hypothesis that maintenance of high levels of IgA in GCF may be “protective” against periodontal attachment loss. Furthermore, levels of β‐glucuronidase appear to be a more sensitive indicator of gingival inflammation than clinical measures. J Periodontol 1999;70:1221‐1227.