Active human hepatitis B viral polymerase expressed in rabbit reticulocyte lysate system

• Published in: Virus genes 1999, Vol.19 (2), p.123-130
• Main Authors: Kim, Y, Jung, G
• Format: Article
• Language: English
• Subjects: Animals; DNA-Directed DNA Polymerase - genetics; DNA-Directed DNA Polymerase - metabolism; Gene Products, pol - genetics; Gene Products, pol - metabolism; Hepatitis B; Hepatitis B virus; Hepatitis B virus - enzymology; magnesium chloride; manganese chloride; P protein; potassium chloride; Protein Biosynthesis; Rabbits; Reticulocytes; RNA-Directed DNA Polymerase - genetics; RNA-Directed DNA Polymerase - metabolism; sodium chloride; Transcription, Genetic
• ISSN: 0920-8569; 1572-994X
• DOI: 10.1023/A:1008175107309

Human HBV polymerase has been expressed in reticulocyte lysate system. The expressed protein shows the DNA-dependent DNA polymerase activity. In vitro transcription and translation produces a major protein product with an apparent molecular weight of approximately 100 kD. The HBV DNA polymerase has been characterized biochemically in the condition that the contaminating cellular DNA polymerases were fairly suppressed by aphidicolin and NEM. The polymerization reaction is optimal at pH 7.5 and 37 degrees C and the polymerase requires either MnCl2 or MgCl2, with a preference for MnCl2. The protein represented an optimal activity in the presence of either 75 mM NaCl or 100 mM KCl, with a higher activity at 75 mM NaCl than 100 mM KCl. Study of the polymerizing activity of the deleted versions of the polymerase protein suggests that the terminal protein is essential for full polymerase function and the spacer region may decrease the stability of the P protein.