Identification of LDL-Receptor Transcription Stimulating Peptides from Soybean Hydrolysate in Human Hepatocytes

Soybean protein and its hydrolysate have been reported to have cholesterol-lowering property, but the responsible components are still largely unknown. In previous study, we found that soybean protein hydrolysate (SPH) prepared with the protease from Bacillus amyloliquefaciens FSE-68, strongly stimu...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2008-06, Vol.56 (12), p.4372-4376
Main Authors: Cho, Seong-Jun, Juillerat, Marcel A, Lee, Cherl-Ho
Format: Article
Language:English
Subjects:
Bacillus - enzymology
Bacillus amyloliquefaciens
Bioactive Constituents
bioactive peptide
Biological and medical sciences
Cell Line
cholesteremic effect
cholesterol
chromatography
cultured cells
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
gene expression
hepatocytes
Hepatocytes - chemistry
human cell lines
Humans
Hydrolysis
in vitro studies
LDL-receptor
low density lipoprotein
Peptide Hydrolases - metabolism
peptides
Peptides - analysis
Peptides - chemistry
Peptides - pharmacology
protein hydrolysates
receptors
Receptors, LDL - genetics
soy protein
Soybean
Soybean Proteins - chemistry
Soybean Proteins - metabolism
transcription (genetics)
Transcription, Genetic - drug effects
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Summary:Soybean protein and its hydrolysate have been reported to have cholesterol-lowering property, but the responsible components are still largely unknown. In previous study, we found that soybean protein hydrolysate (SPH) prepared with the protease from Bacillus amyloliquefaciens FSE-68, strongly stimulates transcription of low density lipoprotein receptor (LDL-R). To identify LDL-R transcription stimulating peptides in human hepatocytes, the SPH was fractionated with gel permeation chromatograpy and the active fraction was further separated by using reverse-phase chromatography. Several peptides in the most active fraction were identified by LC/MS and MS/MS analysis. LDL-R transcription stimulating peptides were synthesized on the basis of identified sequences, and their effect on LDL-R transcription was tested in vitro. Among the synthesized peptides, Phe-Val-Val-Asn-Ala-Thr-Ser-Asn (FVVNATSN) showed the strongest activity, and LDL-R transcription of hepatic cells was increased to 248.8% (compared to 100% of untreated control) by FVVNATSN at a concentration of 100 µM. This study provides direct evidence that peptides derived from soybean protein can influence LDL-R transcription in hepatocytes.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf800676a