The culture site of the gallbladder affects recovery of bacteria in symptomatic cholelithiasis

Traditional methods for bile culturing may miss a large number of underlying bacterial infections that could lead to acute or chronic cholecystitis. to evaluate possible differences regarding the site of material collection and thus to detect the most suitable sample site for gallbladder culture. A...

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Bibliographic Details
Published in:Journal of gastrointestinal and liver diseases : JGLD 2008-06, Vol.17 (2), p.179-182
Main Authors: Manolis, Evangelos N, Filippou, Dimitrios K, Papadopoulos, Vassilios P, Kaklamanos, Ioannis, Katostaras, Theophanis, Christianakis, Efstratios, Bonatsos, Gerasimos, Tsakris, Athanassios
Format: Article
Language:English
Subjects:
Bacteria - isolation & purification
Bacterial Infections - complications
Bacterial Infections - microbiology
Bile - microbiology
Cholecystectomy, Laparoscopic
Cholecystitis - complications
Cholecystitis - microbiology
Cholecystitis - surgery
Cholelithiasis - complications
Cholelithiasis - pathology
Cholelithiasis - surgery
Colony Count, Microbial
Follow-Up Studies
Gallbladder - microbiology
Gallbladder - pathology
Humans
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Summary:Traditional methods for bile culturing may miss a large number of underlying bacterial infections that could lead to acute or chronic cholecystitis. to evaluate possible differences regarding the site of material collection and thus to detect the most suitable sample site for gallbladder culture. A cohort of 137 patients with symptomatic cholelithiasis was enrolled. After surgical excision of the gallbladder, bile cultures were separately performed from fundus, body and neck. Identification of bacteria as well as computation of mean bacterial concentrations were performed with standard microbiological techniques. Wilcoxon's paired and Chi-square tests were used for comparison between continuous and discrete parameters, respectively. Thirty-one patients (22.6%) demonstrated at least one positive culture sample. Positivity was 31/31 (100.0%) in neck samples, 20/31 (64.5%) in body and 13/31 (41.9%) in fundus samples (P
ISSN:1841-8724