HL-60 Differentiating Activity and Flavonoid Content of the Readily Extractable Fraction Prepared from Citrus Juices

Citrus plants are rich sources of various bioactive flavonoids. To eliminate masking effects caused by hesperidin, naringin, and neoeriocitrin, the abundant flavonoid glycosides which make up 90% of the conventionally prepared sample, the readily extractable fraction from Citrus juice was prepared b...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1999-01, Vol.47 (1), p.128-135
Main Authors: Kawaii, Satoru, Tomono, Yasuhiko, Katase, Eriko, Ogawa, Kazunori, Yano, Masamichi
Format: Article
Language:English
Subjects:
antineoplastic agents
Biological and medical sciences
Cell Differentiation - drug effects
chemical composition
Citrus
Citrus - chemistry
flavonoids
Flavonoids - analysis
Flavonoids - pharmacology
fruit juices
General pharmacology
HL-60 Cells
Humans
Magnetic Resonance Spectroscopy
Mass Spectrometry
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Extracts - pharmacology
quantitative analysis
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Summary:Citrus plants are rich sources of various bioactive flavonoids. To eliminate masking effects caused by hesperidin, naringin, and neoeriocitrin, the abundant flavonoid glycosides which make up 90% of the conventionally prepared sample, the readily extractable fraction from Citrus juice was prepared by adsorbing on HP-20 resin and eluting with EtOH and acetone from the resin and was subjected to HL-60 differentiation assay and quantitative analysis of major flavonoids. Screening of 34 Citrus juices indicated that King (C. nobilis) had a potent activity for inducing differentiation of HL-60, and the active principles were isolated and identified as four polymethoxylated flavonoids, namely, nobiletin, 3,3‘,4‘,5,6,7,8-heptamethoxyflavone, natsudaidain, and tangeretin. HPLC analysis of the readily extractable fraction also indicated that King contained high amounts of these polymethoxylated flavonoids among the Citrus juices examined. Principal component and cluster analyses of the readily extractable flavonoids indicated peculiarities of King and Bergamot. Keywords: Citrus; flavonoids; HPLC; multivariate analysis; differentiation; HL-60
ISSN:0021-8561
1520-5118
DOI:10.1021/jf9805101