Localization of the cGMP-dependent protein kinases in relation to nitric oxide synthase in the brain

The distributions of the type I and type II isoforms of cGMP-dependent protein kinase were determined in the rat brain using immunohistochemistry and in situ hybridization, and compared with the localization of NO synthase determined with NADPH-diaphorase histochemistry. The type I cGMP-dependent pr...

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Bibliographic Details
Published in:Journal of chemical neuroanatomy 1999-09, Vol.17 (1), p.45-55
Main Authors: El-Husseini, A.E.-D, Williams, J, Reiner, P.B, Pelech, S, Vincent, S.R
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Brain - cytology
Brain - enzymology
Cerebellar Cortex - enzymology
Cyclic GMP
Cyclic GMP-Dependent Protein Kinases - metabolism
Immunoenzyme Techniques
Immunohistochemistry
In Situ Hybridization
NADPH-diaphorase
Nitric oxide
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type I
Protein kinase
protein kinase G
Purkinje Cells - enzymology
Rabbits
Rats
Rats, Wistar
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Summary:The distributions of the type I and type II isoforms of cGMP-dependent protein kinase were determined in the rat brain using immunohistochemistry and in situ hybridization, and compared with the localization of NO synthase determined with NADPH-diaphorase histochemistry. The type I cGMP-dependent protein kinase was highly expressed in the Purkinje cells of the cerebellar cortex, where it was closely associated with the NO synthase containing granule and basket cells. This kinase was also found in neurons in the dorsomedial nucleus of the hypothalamus, where it may be regulated by NO or atriopeptides. The type I kinase was not detected in other central neurons. In contrast, the type II kinase was widely distributed in the brain. In particular, it was highly expressed in the olfactory bulb, cortex, septum, thalamus, tectum and various brainstem nuclei. Many regions expressing this kinase also contained, or received innervation from NO synthase positive neurons. These results indicate that type I cGMP-dependent protein kinase may act as a downstream effector for NO only in the cerebellar cortex and the dorsomedial hypothalamus. The type II cGMP-dependent protein kinase appears to be a major mediator of NO actions in the brain.
ISSN:0891-0618
1873-6300
DOI:10.1016/S0891-0618(99)00023-X