A development of LC–MS method combining ultrafiltration and lyophilization for determination of r-RGD-Hirudin in human serum

A reliable and validated LC–MS method was established for determination of r-RGD-Hirudin in human serum. Ultrafiltration was used instead of liquid–liquid extraction or solid phase extraction for water solubility drug r-RGD-Hirudin extraction. Freeze drying was used for concentration. The experiment...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2008-07, Vol.870 (1), p.27-31
Main Authors: Su, Shengmin, Yu, Yunqiu, Mo, Wei, Zhang, Yanling, Song, Houyan, Chen, Qinfen, Xie, Yi
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, Liquid - methods
Clinical research
Drug Tolerance
Freeze drying
Freeze Drying - methods
Fundamental and applied biological sciences. Psychology
General pharmacology
Hirudins - administration & dosage
Hirudins - blood
Hirudins - chemistry
Humans
LC–MS
Mass Spectrometry - methods
Medical sciences
Pharmacology. Drug treatments
Quantitative assay
r-RGD-Hirudin
Recombinant Proteins - blood
Recombinant Proteins - chemistry
Sensitivity and Specificity
Ultrafiltration
Ultrafiltration - methods
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Summary:A reliable and validated LC–MS method was established for determination of r-RGD-Hirudin in human serum. Ultrafiltration was used instead of liquid–liquid extraction or solid phase extraction for water solubility drug r-RGD-Hirudin extraction. Freeze drying was used for concentration. The experiment conditions, including pre-processing procedure and LC–MS, have been investigated and optimized. Comparing with reported assays, the current method showed significant improvement in specificity, linearity, precision and sensitivity. This method has been successfully applied in clinical research of r-RGD-Hirudin.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2008.05.032