Determination of plasma topiramate concentration using LC-MS/MS for pharmacokinetic and bioequivalence studies in healthy Korean volunteers

• Published in: Biomedical chromatography 2008-08, Vol.22 (8), p.822-829
• Main Authors: Park, Jin-Hee, Park, Yoo-Sin, Lee, Min-Ho, Rhim, Si-Youn, Song, Jae-Chul, Lee, Soo-Jin, Kim, Jung-Mogg, Shaw, Leslie M, Kang, Ju-Seop
• Format: Article
• Language: English
• Subjects: Anticonvulsants - blood; Anticonvulsants - pharmacokinetics; bioequivalence study; Calibration; Chromatography, Liquid - methods; Fructose - analogs & derivatives; Fructose - blood; Fructose - pharmacokinetics; Humans; Korea; LC-MS/MS; pharmacokinetics; Reference Values; Tandem Mass Spectrometry - methods; Therapeutic Equivalency; topiramate
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.995

A rapid, simple and validated liquid chromatography coupled to tandem mass spectrometric method (LC‐MS/MS) for topiramate analysis in human plasma has been applied to pharmacokinetic and bioequivalence studies in 24 healthy male Korean volunteers. The procedure involves a simple liquid extraction of topiramate and prednisone (internal standard) with acetonitrile and separation by HPLC equipped with a Capcell Pak C18 column using acetonitrile–0.1% triethylamine (80:20, v/v) as a mobile phase. Detection was carried out on an API 2000 MS system by multiple reactions monitoring mode. The ionization was optimized using ESI(−) and selectivity was achieved by MS/MS analysis, m/z 338.0 → 77.5 and m/z 357.1 → 327.2 for topiramate and prednisone, respectively. The method had a total run time of 2.5 min and showed good linearity over a working range of 20–5000 ng/mL in human plasma with a lower limit of quantification of 20 ng/mL. No metabolic compounds were found to interfere with the analysis. The inter‐day and intra‐day accuracy were in the ranges of 99.24–116.63 and 93.45–108.68%, respectively, and inter‐day and intra‐day precisions were below 6.24 and 5.25%, respectively. This method was successfully applied for pharmacokinetic and bioequivalence studies by analysis of blood samples taken up to 96 h after an oral administration of 100 mg of topiramate in 24 healthy Korean volunteers. Copyright © 2008 John Wiley & Sons, Ltd.