Cloning and characterization of a human GDPD domain-containing protein GDPD5

Glycerophosphodiester phosphodiesterase (GDPD) catalyzes the hydrolysis of deacylated glycerophospholipids to glycerol phosphate and alcohol. GDPD5 has been reported in Mus musculus and Gallus gallus, but not in Homo sapiens. Here we report the cloning and characterization of a novel human GDPD doma...

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Bibliographic Details
Published in:Molecular biology reports 2008-09, Vol.35 (3), p.351-359
Main Authors: Lang, Qingyu, Zhang, Haoxing, Li, Jie, Yin, Hongkun, Zhang, Yifeng, Tang, Wenwen, Wan, Bo, Yu, Long
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animal Anatomy
Animal Biochemistry
Animals
Biomedical and Life Sciences
Cell Line
Chromosomes - genetics
Cloning, Molecular
Cytoplasm
GDPD domain
Gene expression
Gene Expression Regulation, Enzymologic
Genome - genetics
Glycerophosphodiester phosphodiesterase
Histology
Humans
Life Sciences
Lipids
Molecular biology
Molecular Sequence Data
Morphology
Organ Specificity
Phosphoric Diester Hydrolases - chemistry
Phosphoric Diester Hydrolases - genetics
Phosphoric Diester Hydrolases - metabolism
Phylogeny
Proteins
Sequence Alignment
Sequence Homology, Amino Acid
Signal transduction
SRE
Transcriptional Activation - genetics
Transmembrane
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Summary:Glycerophosphodiester phosphodiesterase (GDPD) catalyzes the hydrolysis of deacylated glycerophospholipids to glycerol phosphate and alcohol. GDPD5 has been reported in Mus musculus and Gallus gallus, but not in Homo sapiens. Here we report the cloning and characterization of a novel human GDPD domain-containing gene, GDPD5, isolated from human testis cDNA library, and mapped to 11q13.4-13.5 by searching the UCSC genomic database. The GDPD5 cDNA sequence of 3442 base pairs contains an open reading frame encoding 605 amino acids. The GDPD5 gene consists of 17 exons and encodes a putative protein with six transmembrane regions and a GDPD motif. Subcellular localization of GDPD5 demonstrated that the protein was localized in the cytoplasm when overexpressed in COS-7 cells. RT-PCR analysis showed that GDPD5 was widely expressed in human tissues and the expression levels in kidney and prostate were relatively low.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-007-9093-3