One-chip sensing device (biomedical photonic LSI) enabled to assess hippocampal steep and gradual up-regulated proteolytic activities

We developed an implantable one-chip biofluoroimaging device (termed biomedical photonic LSI; BpLSI) which enabled real-time molecular imaging with conventional electrophysiology in vivo in deep brain areas. The multimodal LSI enabled long-term sequential imaging of the fluorescence emitted by prote...

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Bibliographic Details
Published in:Journal of neuroscience methods 2008-08, Vol.173 (1), p.114-120
Main Authors: Tamura, Hideki, Ng, David C., Tokuda, Takashi, Naoki, Honda, Nakagawa, Takuma, Mizuno, Taro, Hatanaka, Yumiko, Ishikawa, Yasuyuki, Ohta, Jun, Shiosaka, Sadao
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Biomedical Technology - instrumentation
CMOS
Coumarins - pharmacology
Electrophysiology - instrumentation
Electrophysiology - methods
Excitatory Postsynaptic Potentials - drug effects
Excitatory Postsynaptic Potentials - physiology
Excitatory Postsynaptic Potentials - radiation effects
Fluorescence
Hippocampus - drug effects
Hippocampus - physiology
Imaging
Kallikrein
Kallikreins - metabolism
Long-Term Potentiation - drug effects
Long-Term Potentiation - physiology
LTP
Male
Mice
Models, Biological
Oligopeptides - pharmacology
Plasticity
Proteolysis
Serine Endopeptidases - metabolism
Serine protease
Time Factors
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Summary:We developed an implantable one-chip biofluoroimaging device (termed biomedical photonic LSI; BpLSI) which enabled real-time molecular imaging with conventional electrophysiology in vivo in deep brain areas. The multimodal LSI enabled long-term sequential imaging of the fluorescence emitted by proteolysis-linked fluorogenic substrate. Using the BpLSI, we observed a process of stimulation-dependent modulation at synapse with multi-site (16×19 pixel) in widespread area and a high-speed video rate, and found that the gradual up-regulated proteolytic activity in a wide range of hippocampal CA1 area and the steep activity in local area, indicating that the proteolysis system is a basis for the fixation of long-term potentiation in post-excited synapses in the hippocampus. Mathematical data analysis confirmed the direct involvement of functional proteolysis for neural plasticity.
ISSN:0165-0270
1872-678X
DOI:10.1016/j.jneumeth.2008.06.002