The macrophage system in the intestinal muscularis externa during inflammation: an immunohistochemical and quantitative study of osteopetrotic mice

Intestinal inflammation results in disturbed intestinal motility in humans as well as in animal models. This altered function of smooth muscle cells and/or the enteric nervous system may be caused by activation of macrophages in muscularis externa and a thereby following release of cytokines and che...

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Bibliographic Details
Published in:Histochemistry and cell biology 2008-08, Vol.130 (2), p.363-373
Main Authors: Mikkelsen, H. B., Larsen, J. O., Hadberg, H.
Format: Article
Language:English
Subjects:
Animals
Antigens, Differentiation - immunology
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cellular biology
Cytokines
Developmental Biology
Female
Gastroenterology
Histocompatibility Antigens Class II - immunology
Inflammation - immunology
Inflammation - pathology
Inflammatory diseases
Jejunum - immunology
Jejunum - pathology
Lipopolysaccharides - immunology
Macrophage Activation - immunology
Macrophages - immunology
Mice
Mice, Mutant Strains
Muscle, Smooth - immunology
Muscle, Smooth - pathology
Original Paper
Osteopetrosis - immunology
Rodents
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Summary:Intestinal inflammation results in disturbed intestinal motility in humans as well as in animal models. This altered function of smooth muscle cells and/or the enteric nervous system may be caused by activation of macrophages in muscularis externa and a thereby following release of cytokines and chemokines that causes influx of mononuclear cells and neutrophilic granulocytes. We subjected osteopetrotic ( op / op ) mice that lack certain macrophage subtypes, e.g. macrophages in the muscularis externa and +/+ mice to LPS to induce inflammatory cell influx. The densities of F4/80 + , MHCII + , and myeloperoxidase + cells were quantified using stereological sampling. In +/+ mice we found that MHCII + cells outnumber F4/80 + cells and that LPS injection increased the density of MHCII + cells temporarily but not that of F4/80 + cells. This indicates that an upregulation of MHCII antigen takes place and that two or more macrophage subtypes with comparable morphologies exist. Osteopetrotic mice lacked MHCII + , CD169 + , and F4/80 + cells after either treatment, which indicate that these cells are CSF-1-dependent. LPS induced VCAM-1 activation of the vessels, modest influx of granulocytes, as well as an iNOS-activation in a cell type different from macrophages in both +/+ and op / op mice.
ISSN:0948-6143
1432-119X
DOI:10.1007/s00418-008-0423-x