In vivo expression of osteogenic markers and bone mineral density at the surface of fluoride-modified titanium implants

Abstract The aim of the present study was to investigate the biological mechanisms of the functional attachment of fluoride-modified titanium implants to cortical bone by studying the association of the pull-out test results with gene expression of osteoblast (runx2, osteocalcin, collagen-I and IGF-...

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Bibliographic Details
Published in:Biomaterials 2008-10, Vol.29 (28), p.3771-3780
Main Authors: Monjo, Marta, Lamolle, Sébastien F, Lyngstadaas, S. Petter, Rønold, H. Jacob, Ellingsen, Jan Eirik
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Biomarkers - metabolism
Bone
Bone Density
Coated Materials, Biocompatible - chemistry
Coated Materials, Biocompatible - metabolism
Dentistry
Female
Fluoride
Fluorides - chemistry
Fluorides - metabolism
Gene expression
Gene Expression Profiling
Humans
Implants, Experimental
In vivo test
Materials Testing
Osseointegration
Osteogenesis - physiology
Rabbits
Surface Properties
Tensile Strength
Tibia - anatomy & histology
Tibia - injuries
Tibia - metabolism
Titanium
Titanium - chemistry
Titanium - metabolism
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Summary:Abstract The aim of the present study was to investigate the biological mechanisms of the functional attachment of fluoride-modified titanium implants to cortical bone by studying the association of the pull-out test results with gene expression of osteoblast (runx2, osteocalcin, collagen-I and IGF-I), osteoclast (TRAP, H+ -ATPase and calcitonin receptor) and inflammation (TNF-α, IL-6 and IL-10) markers from peri-implant bone tissue using real-time RT–PCR, following a 4- and 8-week healing period. After implant detachment, wound fluid from the implant site was collected for LDH and ALP activity analysis. A new method to study volumetric bone mineral density (vBMD) of sub-implant cortical bone was developed using micro-computed tomography. Our results show lower LDH activity and TRAP mRNA levels in fluoride implants after 4 weeks of healing, yet no differences were found either on the pull-out force or expression of bone formation marker genes. After 8 weeks of healing, both pull-out, vBMD and osteocalcin, runx2 and collage type I gene expression were higher in fluoride implants. In conclusion, fluoride-modified implants seem to modulate both inflammation and bone resorption/formation events at the bone–implant interface, suggesting that these biological effects are an intrinsic part of the clinical performance of this surface.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2008.06.001