Obesity promotes injury induced femoral artery thrombosis in mice

• Published in: Thrombosis research 2008-01, Vol.122 (4), p.549-555
• Main Authors: Nagai, Nobuo, Hoylaerts, Marc F, Cleuren, Audrey C.A, Van Vlijmen, Bart J.M, Lijnen, H. Roger
• Format: Article
• Language: English
• Subjects: Animal model; Animals; Antithrombins - metabolism; Biological and medical sciences; Blood and lymphatic vessels; Blood Coagulation; Body Weight; Cardiology. Vascular system; Chlorides; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous; Factor V - metabolism; Femoral Artery - pathology; Ferric Compounds - pharmacology; Hematology, Oncology and Palliative Medicine; Humans; Male; Medical sciences; Mice; Models, Statistical; Obesity; Obesity - blood; Obesity - complications; Plasminogen Activator Inhibitor 1 - blood; Risk; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Thrombosis; Thrombosis - blood; Thrombosis - complications; Vascular surgery: aorta, extremities, vena cava. Surgery of the lymphatic vessels
• ISSN: 0049-3848; 1879-2472
• DOI: 10.1016/j.thromres.2007.12.017

Abstract An FeCl3 induced femoral arterial thrombosis model was applied to lean (47 ± 1.4 g) and obese (64 ± 1.7 g) mice (Swiss genetic background) in order to study the relation between obesity and thrombotic risk. As compared to lean mice, obese mice showed a significantly shorter occlusion time (9.9 ± 1.0 min versus 13 ± 0.5 min; p = 0.04) and lower total blood flow (37 ± 7.3% versus 69 ± 6.7%; p = 0.008). A significant negative correlation was observed between body weight and both occlusion time ( r = − 0.57; p = 0.014) and blood flow ( r = − 0.57; p = 0.028). Analysis of the coagulation profile revealed significantly higher levels of plasminogen activator inhibitor-1 (PAI-1), thrombin–antithrombin complex, Factor V activity and combined Factors II/VII/X activity, and moderately elevated Factor VIII activity in obese mice. The degree of arterial damage and the thrombus extension were, however, not significantly different. A significant positive correlation was observed between body weight and either PAI-1 ( r = 0.63; p = 0.003), Factors II/VII/X levels ( r = 0.80; p < 0.0001) or Factor V levels ( r = 0.65; p = 0.003). Thus, this injury induced femoral artery thrombosis model in mice establishes experimentally a correlation between obesity and prothrombotic tendency.