Chronic Renal Failure: Leukocyte Rheology and Polymorphonuclear Cytosolic Ca2+ Concentration

Summary In a group of subjects with chronic renal failure (CRF), we evaluated the leukocyte rheological activity, expressed as the filtration rate, the polymorphonuclear (PMN) membrane fluidity and the PMN cytosolic Ca2+ content. Using the St. George's Piltrometer, we determined the filtration...

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Bibliographic Details
Published in:Current medical research and opinion 1999, Vol.15 (3), p.202-207
Main Authors: Lo Presti, R., Canino, B., Vaccaro, F., Montana, M., Ventimiglia, G., Grifò, G., Caimi, G.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Calcium - blood
Case-Control Studies
Chronic renal failure
Cytosol - chemistry
Female
Hemorheology
Humans
Kidney Failure, Chronic - physiopathology
Leukocyte filtration parameters
Leukocytes - cytology
Linear Models
Male
Medical sciences
Membrane Fluidity
Middle Aged
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
Neutrophils - metabolism
Polymorphonuclear cytosolic Ca
Polymorphonuclear membrane fluidity
Renal failure
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Summary:Summary In a group of subjects with chronic renal failure (CRF), we evaluated the leukocyte rheological activity, expressed as the filtration rate, the polymorphonuclear (PMN) membrane fluidity and the PMN cytosolic Ca2+ content. Using the St. George's Piltrometer, we determined the filtration rate of unfractionated, mononuclear and PMN leukocytes. Using the fluorescent probe 1.4-(trimethylamino)-phenyl-4-phenyl-hexatriene (TMA-DPH), we examined the PMN membrane fluidity and, using the Fura 2-AM, the PMN cytosolic Ca2+ content. Prom the results obtained, it is evident that only the initial relative flow rate of unfractionated leukocytes was significantly reduced in subjects with CRF, while the filtration parameters of mononuclear and PMN cells did not distinguish normals from CRF subjects. No variation was evident for the PMN membrane fluidity, while the PMN cytosolic Ca2+ content was significantly increased in CRF subjects. In these subjects no correlation was found between leukocyte filtration parameters, PMN membrane fluidity, PMN cytosolic Ca2+ content and plasma parameters (blood urea nitrogen and serum creatinine), reflecting the degree of the CRF. In conclusion, in CRF subjects the abnormality of the leukocyte flow properties was restricted to the initial flow rate of unfractionated leukocytes, whereas the increase of PMN cytosolic Ca2+ content might be attributed to PMN activation.
ISSN:0300-7995
1473-4877
DOI:10.1185/03007999909114092