Domains of differential cell proliferation suggest hinged folding in avian gut endoderm

A profile of proliferative growth assessed with tritium autoradiograms from White Leghorn embryo stages Hamburger–Hamilton 6–21 labeled in ovo presents evidence of hinged folding driven by localized differential cell proliferation in endoderm. There is a significant, bilateral pattern, and differenc...

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Bibliographic Details
Published in:Developmental dynamics 1999-12, Vol.216 (4‐5), p.398-410
Main Authors: Miller, Sue Ann, Adornato, Michael, Briglin, Ailish, Cavanaugh, Megan, Christian, Todd, Jewett, Kelly, Michaelson, Cheryl, Monoson, Tama, Price, Frank, Tignor, Julie, Tyrell, Dean
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Autoradiography
Body Patterning
Cell Differentiation
cell proliferation
chick embryo
Chick Embryo - physiology
Digestive System - embryology
endoderm
Endoderm - cytology
Endoderm - physiology
epithelium
Mitotic Index
morphogenesis
Thymidine - metabolism
Tritium
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Summary:A profile of proliferative growth assessed with tritium autoradiograms from White Leghorn embryo stages Hamburger–Hamilton 6–21 labeled in ovo presents evidence of hinged folding driven by localized differential cell proliferation in endoderm. There is a significant, bilateral pattern, and differences are most pronounced in axial levels that are folding and rotating. Highest proliferation is in cells producing folds; lowest proliferation is in median cells. Localized changes in cell shape are lacking, as are TUNEL markers and cell morphology that would suggest involvement of apoptosis. Folding endoderm to form gut tube appears to be a process that is driven by domains of high cell proliferation flanking a domain of significantly lower proliferation. When considered in the context of an epithelium attached to subjacent mesoderm, these differentials could produce a forced and directed buckling of endoderm into lateral folds that join and enclose a tube. Patterns suggest that endoderm folds about a median hinge. In the light of this new information, we suggest it is more precise to refine the term, median hinge point (MHP), to neural hinge point (NHP) and gut hinge point (GHP). Dev Dyn 1999;216:398–410. ©1999 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/(SICI)1097-0177(199912)216:4/5<398::AID-DVDY8>3.0.CO;2-7