Antigen phenotype of cultured decidual stromal cells of human term decidua

We previously reported that decidual stromal cells (DSC) from early human decidua express antigens associated with hematopoietic cells and develop different immune functions. Here we study the antigenic phenotype of DSC from term decidua and compare it with the phenotype reported for DSC from early...

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Bibliographic Details
Published in:Journal of reproductive immunology 1999-11, Vol.45 (1), p.19-30
Main Authors: Oliver, Carmen, Cowdrey, Nina, Abadı́a-Molina, Ana C., Olivares, Enrique G.
Format: Article
Language:English
Subjects:
Abortion
Antigens, CD - analysis
B7-1 Antigen - analysis
Biological and medical sciences
Biomarkers
decidua
Decidua - cytology
Decidua - immunology
Decidual stromal cells
Decidualization
Desmin - analysis
Female
Flow Cytometry
Follicular dendritic cells
Fundamental and applied biological sciences. Psychology
Gene expression
HLA-DR Antigens - analysis
Human pregnancy
Humans
Immunophenotyping
Labor, Obstetric
Molecular and cellular biology
Molecular genetics
Neprilysin - analysis
Pregnancy
Pregnancy Trimester, First
Pregnancy Trimester, Third
Prolactin - analysis
Receptors, Complement 3d - analysis
Receptors, IgE - analysis
Stromal Cells - immunology
Vimentin - analysis
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Summary:We previously reported that decidual stromal cells (DSC) from early human decidua express antigens associated with hematopoietic cells and develop different immune functions. Here we study the antigenic phenotype of DSC from term decidua and compare it with the phenotype reported for DSC from early decidua. Decidual stromal cells were isolated from human term deciduas and maintained in culture until highly purified DSC cultures were obtained. Most term DSC, like most early DSC, expressed CD10. Term DSC expressed antigens specific for follicular dendritic cells (FDC), such as DRC-1 (CD21L) and HJ2, together with CD21, CD23 and CD80, which are detected on FDC as well. Also like early DSC, term DSC were negative for CD3, CD14, CD15 and CD45. Although early DSC were reported to be HLA-DR-positive and CD86-positive, these antigens were not expressed by term DSC. These discrepant results suggest that two types of cells, or cells at different stages of differentiation (decidualization) were selected during culture of decidual cells from different periods of gestation. This possibility was further supported by the finding that term DSC expressed desmin and prolactin, two markers of decidualization, whereas these molecules have not previously been detected in early DSC.
ISSN:0165-0378
1872-7603
DOI:10.1016/S0165-0378(99)00041-8