In vitro fertilization with flow-cytometrically-sorted bovine sperm

An attractive feature of IVF is that fewer sexed sperm are needed than for artificial insemination. However, sperm sexed by flow cytometry/cell sorting are probably precapacitated, necessitating modifications to standard IVF systems for optimal success. With current procedures, the percentages of oo...

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Bibliographic Details
Published in:Theriogenology 1999-12, Vol.52 (8), p.1393-1405
Main Authors: Lu, K.H., Cran, D.G., Seidel, G.E.
Format: Article
Language:English
Subjects:
Animals
Blastocyst - cytology
Cattle
Cell Division
Cell Separation - methods
cytometry
Embryonic and Fetal Development
Female
Fertilization in Vitro - veterinary
Flow Cytometry
IVF
Male
Oocytes - cytology
Oocytes - physiology
Ovarian Follicle - cytology
Sex Determination Analysis
sexing
sperm
Spermatozoa - cytology
X Chromosome
Y Chromosome
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Summary:An attractive feature of IVF is that fewer sexed sperm are needed than for artificial insemination. However, sperm sexed by flow cytometry/cell sorting are probably precapacitated, necessitating modifications to standard IVF systems for optimal success. With current procedures, the percentages of oocytes fertilized with sorted and unsorted frozen bovine sperm are similar, and events during the first cell cycle are timed similarly for sorted and unsorted sperm. However, in most cases, blastocyst production with sorted sperm was ~70% of controls produced with unsorted sperm. In some early studies, there appeared to be an unexplained delay of about half a day in blastocyst development. Nevertheless, some dozens of apparently normal calves, pre-sexed with 90% accuracy, have resulted from frozen embryos produced via IVF with sexed sperm. IVF also has proven useful as a bioassay for improving sperm-sorting procedures such as determining potential detrimental effects of laser power. It is likely that use of IVF in cattle breeding programs will increase considerably when sexed, frozen sperm become commercially available.
ISSN:0093-691X
1879-3231
DOI:10.1016/S0093-691X(99)00225-3