Experimental conditions that increase the production of HIV-1 by monocyte-derived macrophages: use of collagen matrix

Monocyte-derived macrophages (MDMs) from healthy blood donors were isolated by adherence to tissue culture-treated plasticware. They were cultured in vitro in medium supplemented with human serum and recombinant GM-CSF, then infected with the macrophage-tropic prototype strain HIV-1-PAR. Virus produ...

Full description

Saved in:
Bibliographic Details
Published in:Microbes and infection 1999-08, Vol.1 (10), p.765-770
Main Authors: Godard, Chantal M, Chermann, Jean-Claude
Format: Article
Language:English
Subjects:
AIDS/HIV
Biological and medical sciences
Blood Proteins - pharmacology
Cell Separation
collagen
Collagen - pharmacology
collagen 1
extracellular matrix
Fundamental and applied biological sciences. Psychology
Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology
HIV-1 - growth & development
HIV-1 - immunology
HIV-1 replication
human immunodeficiency virus 1
Humans
In Vitro Techniques
Macrophages - immunology
Macrophages - virology
Microbiology
monocyte-macrophage
Monocytes
Plastics - pharmacology
Recombinant Proteins
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Time Factors
Virology
Virus Cultivation - methods
Virus Replication - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Monocyte-derived macrophages (MDMs) from healthy blood donors were isolated by adherence to tissue culture-treated plasticware. They were cultured in vitro in medium supplemented with human serum and recombinant GM-CSF, then infected with the macrophage-tropic prototype strain HIV-1-PAR. Virus production was quantitated at various times after infection by measuring reverse transcriptase concentration in cell-free tissue culture supernatant fluids, using a sensitive nonradioactive assay. Virus production was significantly increased by culturing MDMs on plasticware previously coated with collagen 1. The increase in virus production was dependent upon collagen 1 concentration, with maximal value being encountered after coating with 1.5 μg/cm 2. These results indicate that the sensitivity of peripheral macrophages to HIV-1 infection might be influenced by contact-dependent interactions involving components of the extracellular matrix that take place during the process of monocyte extravasation and migration.
ISSN:1286-4579
1769-714X
DOI:10.1016/S1286-4579(99)80078-1