Molecular typing of Mycobacterium tuberculosis isolates from a rural area of Kanpur by spoligotyping and mycobacterial interspersed repetitive units (MIRUs) typing

Molecular typing of Mycobacterium tuberculosis isolates has greatly facilitated the understanding of epidemiology of tuberculosis (TB). This study was done to characterize prevalent genotypes of M. tuberculosis on a collection of 97 isolates based on spoligotyping and mycobacterial interspersed repe...

Full description

Saved in:
Bibliographic Details
Published in:Infection, genetics and evolution genetics and evolution, 2008-09, Vol.8 (5), p.621-626
Main Authors: Sharma, Pragya, Chauhan, Devendra Singh, Upadhyay, Prashant, Faujdar, Jaya, Lavania, Mallika, Sachan, Shailender, Katoch, Kiran, Katoch, Vishwa Mohan
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Bacterial Typing Techniques
Child
Female
Genetic Variation
Genotype
Humans
India - epidemiology
Interspersed Repetitive Sequences - genetics
Loci
Male
Middle Aged
MIRU-VNTR
Mycobacterium tuberculosis
Mycobacterium tuberculosis - classification
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Phylogeny
Rural Population
Shared type
Spoligotyping
Tuberculosis - epidemiology
Tuberculosis - microbiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Molecular typing of Mycobacterium tuberculosis isolates has greatly facilitated the understanding of epidemiology of tuberculosis (TB). This study was done to characterize prevalent genotypes of M. tuberculosis on a collection of 97 isolates based on spoligotyping and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) typing in rural area of Kanpur, North India. In this area different types of interventions are being undertaken and follow-up studies are progressing. Predominant spoligotypes prevalent in this region belonged to Central Asian–Delhi family (CAS1_Del) (37%), East African–Indian family (11%), T1 family (8%) and Beijing (4%) family. Highly distinct MIRU-VNTR genotypes were obtained. Significant spoligotypes such as Beijing and CAS1_Del type were further divided into subtypes with MIRU-VNTR. This preliminary study reveals that CAS is the most predominant family in this rural area of Kanpur. If confirmed in other areas, this combined approach of molecular typing can be preferably be used as first line tool for studying linkage and transmission dynamics of TB in India.
ISSN:1567-1348
1567-7257
DOI:10.1016/j.meegid.2008.05.002