Fluorescence in situ hybridization analysis of the prokaryotic community inhabiting crystallizer ponds

A fluorescence in situ hybridization (FISH) protocol suitable for the identification of prokaryotes inhabiting hypersaline environments was developed and applied to several crystallizer ponds with salinities above 36% from a multipond solar saltern in Alicante, Spain. Two morphotypes were abundant i...

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Bibliographic Details
Published in:Environmental microbiology 1999-12, Vol.1 (6), p.517-523
Main Authors: Antón, Josefa, Llobet-Brossa, Enric, Rodríguez-Valera, Francisco, Amann, Rudolf
Format: Article
Language:English
Subjects:
Archaea - genetics
Archaea - isolation & purification
DNA Probes
DNA, Archaeal - analysis
DNA, Archaeal - genetics
DNA, Ribosomal - analysis
DNA, Ribosomal - genetics
Haloarcula - isolation & purification
Haloferax - isolation & purification
In Situ Hybridization, Fluorescence
RNA, Ribosomal, 16S - genetics
Sodium Chloride
Water Microbiology
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Summary:A fluorescence in situ hybridization (FISH) protocol suitable for the identification of prokaryotes inhabiting hypersaline environments was developed and applied to several crystallizer ponds with salinities above 36% from a multipond solar saltern in Alicante, Spain. Two morphotypes were abundant in these environments: rods and square or square‐like prokaryotes that could be affiliated to Bacteria and Archaea, respectively, by FISH with domain‐specific probes. FISH with a newly designed probe proved that the archaeal 16S rDNA sequence most frequently recovered from the crystallizers, SPhT, originated from the dominant square‐like prokaryotes. These uncultured prokaryotes have the morphology of Walsby's square bacteria. Additionally, FISH with a probe targeted to the genus Haloarcula, members of which are frequently isolated from this environment, indicated that this genus accounts for less than 0.1% of the total prokaryotic community.
ISSN:1462-2912
1462-2920
DOI:10.1046/j.1462-2920.1999.00065.x