Evaluation of quantitative anti-F1 IgG and anti-V IgG ELISAs for use as an in vitro-based potency assay of plague vaccine in mice

Quantitative anti-F1 and anti-V IgG enzyme-linked immunosorbent assays (ELISAs) were developed to measure the serological response of female Swiss Webster mice after vaccination with the recombinant fusion protein, rF1-V, which is being developed as a plague vaccine. Several fundamental parameters o...

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Bibliographic Details
Published in:Biologicals 2008-09, Vol.36 (5), p.287-295
Main Authors: Little, S.F., Webster, W.M., Wilhelm, H., Powell, B., Enama, J., Adamovicz, J.J.
Format: Article
Language:English
Subjects:
Animals
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Female
Immunoglobulin G - immunology
Mice
Plague
Plague Vaccine - immunology
Plague Vaccine - standards
Potency assay
Recombinant F1-V vaccine
Reference Standards
Reproducibility of Results
Substrate Specificity
Yersinia pestis
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Summary:Quantitative anti-F1 and anti-V IgG enzyme-linked immunosorbent assays (ELISAs) were developed to measure the serological response of female Swiss Webster mice after vaccination with the recombinant fusion protein, rF1-V, which is being developed as a plague vaccine. Several fundamental parameters of the ELISA were evaluated: specificity, precision, accuracy, and stability. Experimental results suggested that a potency assay based upon the serological response of female Swiss Webster mice, as measured by quantitative anti-F1 IgG and anti-V IgG ELISAs, might be used to evaluate the rF1-V fusion protein vaccine.
ISSN:1045-1056
1095-8320
DOI:10.1016/j.biologicals.2008.03.001