Preparation and cytocompatibility of PLGA scaffolds with controllable fiber morphology and diameter using electrospinning method

Poly(lactide‐co‐glycolide) (PLGA) scaffolds prepared by the electrospinning technology were paid more attention in tissue engineering, but detailed investigation on the influence of process parameters on scaffold morphology and fiber diameters as well as distribution remains to be conducted. In the...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2008-10, Vol.87B (1), p.26-34
Main Authors: Zhao, Li, He, Chenguang, Gao, Yongjuan, Cen, Lian, Cui, Lei, Cao, Yilin
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemistry
Cell Adhesion
Cell Proliferation
electrospinning
fibroblasts
Fibroblasts - cytology
Humans
Lactic Acid - chemistry
Lactic Acid - therapeutic use
nanofiber
Nanostructures - chemistry
PLGA scaffolds
Polyglycolic Acid - chemistry
Polyglycolic Acid - therapeutic use
tissue engineering
Tissue Engineering - methods
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Summary:Poly(lactide‐co‐glycolide) (PLGA) scaffolds prepared by the electrospinning technology were paid more attention in tissue engineering, but detailed investigation on the influence of process parameters on scaffold morphology and fiber diameters as well as distribution remains to be conducted. In the current study, PLGA concentration, electric field strength, and feeding rate were varied to prepare different PLGA scaffolds. It was shown that with the varying of the above parameters, the electrospun porous PLGA scaffolds exhibited fibrous morphology either with/out beads or bead‐fiber string. The diameter of nanofibers increased with the PLGA concentration and feeding rate, whereas the electric field strength exerted only minor effects on the average diameter of the nanofibers. The cytocompatibility of the scaffold prepared from optimizing fabrication parameters was evaluated using human fibroblasts. Good cell attachment, fast proliferation rate, characteristic fibroblast morphology with homogeneous distribution was observed on the scaffold. As a whole, this study demonstrated that the fiber morphology and diameter of the electrospun PLGA scaffolds could be tailored by controlling fabrication parameters. And nanofibrous PLGA could act as a biologically preferred scaffold for fibroblasts growth with maintenance of characteristic morphology. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.31060