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Distribution of plasmid-borne stress protein genes in Streptococcus thermophilus and other lactic acid bacteria
The presence of heat stress protein genes (hsp) was tested by Southern hybridization analysis in total DNA extracts from species of the genus Streptococcus (47 strains), Lactobacillus (34 strains), Lactococcus (24 strains), and Leuconostoc (5 strains). The biotinylated hsp16.4 probe prepared from an...
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| Published in: | Current microbiology 1999, Vol.38 (1), p.43-47 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| container_end_page | 47 |
| container_issue | 1 |
| container_start_page | 43 |
| container_title | Current microbiology |
| container_volume | 38 |
| creator | Somkuti, G.A Steinberg, D.H |
| description | The presence of heat stress protein genes (hsp) was tested by Southern hybridization analysis in total DNA extracts from species of the genus Streptococcus (47 strains), Lactobacillus (34 strains), Lactococcus (24 strains), and Leuconostoc (5 strains). The biotinylated hsp16.4 probe prepared from an ORF2 fragment of pER341 (2.8 kb) tested positively with restricted DNA extracts of seven Streptococcus thermophilus strains and a single strain of Lactococcus lactis subsp. cremoris. In all positive S. thermophilus strains, the hsp was located on plasmids ranging from cat 2.8 kb to 11 kb in size, while hsp was present in a 7.5-kb plasmid in Lactococcus lactis subsp. cremoris. Southern blots with a rep probe showed that all hsp16.4+ plasmids in S. thermophilus strains also shared homology with the replication function (rep) of pER341, suggesting the common origin of these plasmids. |
| doi_str_mv | 10.1007/PL00006770 |
| format | article |
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The biotinylated hsp16.4 probe prepared from an ORF2 fragment of pER341 (2.8 kb) tested positively with restricted DNA extracts of seven Streptococcus thermophilus strains and a single strain of Lactococcus lactis subsp. cremoris. In all positive S. thermophilus strains, the hsp was located on plasmids ranging from cat 2.8 kb to 11 kb in size, while hsp was present in a 7.5-kb plasmid in Lactococcus lactis subsp. cremoris. 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Psychology ; Genes ; Genes, Bacterial ; Heat tolerance ; Heat-Shock Proteins - genetics ; Lactobacillus ; Lactobacillus - genetics ; Lactococcus ; Lactococcus - genetics ; Leuconostoc ; Leuconostoc - genetics ; Microbiology ; Nucleic Acid Hybridization ; Plasmids ; Streptococcus ; Streptococcus - chemistry ; Streptococcus - genetics</subject><ispartof>Current microbiology, 1999, Vol.38 (1), p.43-47</ispartof><rights>1999 INIST-CNRS</rights><rights>Springer-Verlag New York Inc. 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-39719da08b1999bb3621b58be82243a763dc2c8886ecd628b55f24bf5e2151303</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1691787$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9841781$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Somkuti, G.A</creatorcontrib><creatorcontrib>Steinberg, D.H</creatorcontrib><title>Distribution of plasmid-borne stress protein genes in Streptococcus thermophilus and other lactic acid bacteria</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><description>The presence of heat stress protein genes (hsp) was tested by Southern hybridization analysis in total DNA extracts from species of the genus Streptococcus (47 strains), Lactobacillus (34 strains), Lactococcus (24 strains), and Leuconostoc (5 strains). The biotinylated hsp16.4 probe prepared from an ORF2 fragment of pER341 (2.8 kb) tested positively with restricted DNA extracts of seven Streptococcus thermophilus strains and a single strain of Lactococcus lactis subsp. cremoris. In all positive S. thermophilus strains, the hsp was located on plasmids ranging from cat 2.8 kb to 11 kb in size, while hsp was present in a 7.5-kb plasmid in Lactococcus lactis subsp. cremoris. 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Psychology</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Heat tolerance</topic><topic>Heat-Shock Proteins - genetics</topic><topic>Lactobacillus</topic><topic>Lactobacillus - genetics</topic><topic>Lactococcus</topic><topic>Lactococcus - genetics</topic><topic>Leuconostoc</topic><topic>Leuconostoc - genetics</topic><topic>Microbiology</topic><topic>Nucleic Acid Hybridization</topic><topic>Plasmids</topic><topic>Streptococcus</topic><topic>Streptococcus - chemistry</topic><topic>Streptococcus - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Somkuti, G.A</creatorcontrib><creatorcontrib>Steinberg, D.H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest_Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Somkuti, G.A</au><au>Steinberg, D.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of plasmid-borne stress protein genes in Streptococcus thermophilus and other lactic acid bacteria</atitle><jtitle>Current microbiology</jtitle><addtitle>Curr Microbiol</addtitle><date>1999</date><risdate>1999</risdate><volume>38</volume><issue>1</issue><spage>43</spage><epage>47</epage><pages>43-47</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>The presence of heat stress protein genes (hsp) was tested by Southern hybridization analysis in total DNA extracts from species of the genus Streptococcus (47 strains), Lactobacillus (34 strains), Lactococcus (24 strains), and Leuconostoc (5 strains). The biotinylated hsp16.4 probe prepared from an ORF2 fragment of pER341 (2.8 kb) tested positively with restricted DNA extracts of seven Streptococcus thermophilus strains and a single strain of Lactococcus lactis subsp. cremoris. In all positive S. thermophilus strains, the hsp was located on plasmids ranging from cat 2.8 kb to 11 kb in size, while hsp was present in a 7.5-kb plasmid in Lactococcus lactis subsp. cremoris. Southern blots with a rep probe showed that all hsp16.4+ plasmids in S. thermophilus strains also shared homology with the replication function (rep) of pER341, suggesting the common origin of these plasmids.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>9841781</pmid><doi>10.1007/PL00006770</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0343-8651 |
| ispartof | Current microbiology, 1999, Vol.38 (1), p.43-47 |
| issn | 0343-8651 1432-0991 |
| language | eng |
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| subjects | Action of physical and chemical agents on bacteria Bacteria Bacterial Proteins - genetics Bacteriology Biological and medical sciences Blotting, Southern Deoxyribonucleic acid DNA DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology Genes Genes, Bacterial Heat tolerance Heat-Shock Proteins - genetics Lactobacillus Lactobacillus - genetics Lactococcus Lactococcus - genetics Leuconostoc Leuconostoc - genetics Microbiology Nucleic Acid Hybridization Plasmids Streptococcus Streptococcus - chemistry Streptococcus - genetics |
| title | Distribution of plasmid-borne stress protein genes in Streptococcus thermophilus and other lactic acid bacteria |
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