Vaccine potential of a recombinant glutathione S-transferase cloned from Schistosoma haematobium in primates experimentally infected with an homologous challenge

• Published in: Vaccine 1999-01, Vol.17 (4), p.319-326
• Main Authors: Boulanger, Denis, Warter, André, Sellin, Bertrand, Lindner, Véronique, Pierce, Raymond J, Chippaux, Jean-Philippe, Capron, André
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Helminth - biosynthesis; Biological and medical sciences; Cloning, Molecular; Erythrocebus patas; Female; Fundamental and applied biological sciences. Psychology; Glutathione S-transferase; Glutathione Transferase - immunology; Humans; Invertebrates; Nemathelminthia. Plathelmintha; Parasite Egg Count; Primates; Recombinant vaccine; Schistosoma haematobium; Schistosoma haematobium - immunology; Sequence Homology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Synthetic
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(98)00202-3

Patas monkeys were twice immunized with a Schistosoma haematobium-derived recombinant glutathione S-transferase (Sh28GST) then challenged with an homologous calibrated challenge. BCG and Freund's Complete Adjuvant (FCA) were used as adjuvants in two distinct protocols. Specific IgG and IgA antibody responses were intense and homogeneous in the animals receiving Sh28GST in the presence of FCA, whereas BCG could only induce moderate and heterogeneous antibody titres. No significant effect on worm burdens was evidenced 36 weeks post-infection in either group of Sh28GST-immunized animals compared to their matched controls receiving an irrelevant protein. Although not significant, 50% reductions in the numbers of eggs located in all tissues (FCA group) and in the urogenital system (BCG group) were noted. Moreover, the total number of excreted eggs was dramatically diminished by 60% and 77% in the BCG and FCA groups, respectively. These reductions reached 75% and 80% in the urines of vaccinated monkeys. Bladder pathology was also reduced in the animals displaying the lowest urinary egg excretions. There was no clear positive or negative correlate between antibody responses and individual levels of protection. Taken as a whole, our results show that Sh28GST was capable of significantly reducing S. haematobium worm fecundity in experimentally infected primates. Although FCA induced higher levels of protection, the efficacy of BCG as an adjuvant appeared sufficient to justify consideration of the future application of this new formulation as a vaccine against human urogenital schistosomosis.