In situ hybridization: A molecular approach for the diagnosis of the microsporidian parasite Enterocytozoon bieneusi

Microsporidia are emerging as opportunistic pathogens in patients with acquired immunodeficiency syndrome (AIDS). Enterocytozoon bieneusi is the most commonly reported microsporidium that is detected in gastrointestinal specimens. This report describes an in situ hybridization technique with a 30-ba...

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Bibliographic Details
Published in:Human pathology 1999, Vol.30 (1), p.54-58
Main Authors: Velasquez, Jorge N, Carnevale, Silvana, Labbe, Jorge H, Chertcoff, Agustin, Cabrera, Marta G, Oelemann, Walter
Format: Article
Language:English
Subjects:
AIDS-Related Opportunistic Infections - diagnosis
AIDS-Related Opportunistic Infections - parasitology
AIDS/HIV
Animals
Biological and medical sciences
DNA Probes - chemistry
DNA, Protozoan - analysis
Duodenum - parasitology
Duodenum - pathology
Encephalitozoon - genetics
Encephalitozoon - ultrastructure
Encephalitozoonosis - diagnosis
Encephalitozoonosis - parasitology
Enterocytozoon bieneusi
HIV Enteropathy - diagnosis
Humans
Immunoenzyme Techniques
in situ hybridization
In Situ Hybridization - methods
Intestinal Diseases, Parasitic - diagnosis
Intestinal Diseases, Parasitic - parasitology
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
microsporidiosis
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Polymerase Chain Reaction
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Summary:Microsporidia are emerging as opportunistic pathogens in patients with acquired immunodeficiency syndrome (AIDS). Enterocytozoon bieneusi is the most commonly reported microsporidium that is detected in gastrointestinal specimens. This report describes an in situ hybridization technique with a 30-base specific synthetic DNA probe for detection of E. bieneusi by light microscopy. Formalin-fixed paraffin-embedded duodenal biopsy specimens from three patients with AIDS, chronic diarrhea, and E bieneusi infection confirmed by electron microscopy were used in this study. Light microscopic examination after colorimetric detection allowed the identification of different stages of the pathogen's life cycle in the cytoplasm of enterocytes. No cross-reactivity was noted between the probe and human DNA. Our study underscores the applicability of a synthetic-labeled oligonucleotide for the detection and identification of E bieneusi in clinical samples.
ISSN:0046-8177
1532-8392
DOI:10.1016/S0046-8177(99)90300-3