Tissue distribution and excretion of resveratrol in rat after oral administration of Polygonum cuspidatum extract (PCE)

Polygonum cuspidatum extract as a traditional Chinese medicine is extracted from the dried rhizome and root of Polygonum cuspidatum Sieb.et Zucc. Resveratrol is one of its active components. Studies were performed in rats to define the tissue distribution and excretion of resveratrol in urine and bi...

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Bibliographic Details
Published in:Phytomedicine (Stuttgart) 2008-10, Vol.15 (10), p.859-866
Main Authors: Wang, Donggeng, Xu, Yuerong, Liu, Wenying
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Atherosclerosis
Bile - metabolism
Chemical properties
Chromatography, High Pressure Liquid
Drug therapy
Excretion
Fallopia japonica - chemistry
Health aspects
Materia medica, Vegetable
Plant extracts
Plant Extracts - administration & dosage
Plant Extracts - pharmacokinetics
Plant Extracts - urine
Polygonum
Polygonum cuspidatum extract
Rats
Resveratrol
Spectrophotometry, Ultraviolet
Stilbenes - pharmacokinetics
Stilbenes - urine
Tandem Mass Spectrometry
Tissue Distribution
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Summary:Polygonum cuspidatum extract as a traditional Chinese medicine is extracted from the dried rhizome and root of Polygonum cuspidatum Sieb.et Zucc. Resveratrol is one of its active components. Studies were performed in rats to define the tissue distribution and excretion of resveratrol in urine and bile, and to characterize (if possible) any metabolites of resveratrol observed in tissues after ig 20 mg/kg Polygonum cuspidatum extract. For tissue distribution studies, tissues (300 mg) were homogenized and centrifuged with methanol, and metabolites found in selected tissue extract were identified by LC/MS/MS. For urinary and biliary excretion experiments, urine and bile samples were cleaned up by using solid-phase extraction (SPE) with polyamide cartridges. All the concentrations of resveratrol in these biological samples were determined by HPLC with UV detection. After a single oral dose of 20 mg/kg PCE in rats, resveratrol was mainly distributed in stomach, duodenum, liver and kidney with detectable metabolites resveratrol monoglucuronide and resveratrol monosulfate. The majority of the resveratrol was excreted as metabolites, only 0.59% and 0.027% of the dosage were excreted in urine and bile respectively as unchanged drug within 24 h.
ISSN:0944-7113
1618-095X
DOI:10.1016/j.phymed.2008.02.009