Gradient elution capillary electrochromatography and hyphenation with nuclear magnetic resonance

Coupling of gradient capillary electrochromatography (gradient CEC) and capillary zone electrophoresis (CZE) with nuclear magnetic resonance spectroscopy (NMR) was performed using a recently developed capillary NMR interface. This technique was applied for the analysis of pharmaceuticals and food. A...

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Bibliographic Details
Published in:Electrophoresis 1999-01, Vol.20 (1), p.3-8
Main Authors: Gfrörer, Petra, Schewitz, Jens, Pusecker, Klaus, Tseng, Li-Hong, Albert, Klaus, Bayer, Ernst
Format: Article
Language:English
Subjects:
Acetaminophen - analysis
Analgesics, Non-Narcotic - analysis
Aspirin - analysis
Caffeine - analysis
Capillary electrochromatography
Capillary zone electrophoresis
Chromatography, Liquid - methods
Electrophoresis, Capillary - methods
Hyphenation
Magnetic Resonance Spectroscopy - methods
Molecular Structure
Nuclear magnetic resonance spectroscopy
Pharmaceutical analysis
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Summary:Coupling of gradient capillary electrochromatography (gradient CEC) and capillary zone electrophoresis (CZE) with nuclear magnetic resonance spectroscopy (NMR) was performed using a recently developed capillary NMR interface. This technique was applied for the analysis of pharmaceuticals and food. An analgesic was investigated using isocratic and gradient continuous‐flow CEC‐NMR. Comparison of the results demonstrated the superiority of gradient CEC over isocratic CEC. Aspartame and caffeine, both ingredients of soft beverages, were separated and analyzed by continuous flow CZE‐NMR. The order of elution could be reversed by altering the pH. This reversal led to an increased sample concentration in the NMR detection cell, thus allowing the acquisition of a totally correlated spectroscopy (TOCSY) two‐dimensional (2‐D) spectrum of the synthetic peptide aspartame.
ISSN:0173-0835
1522-2683
DOI:10.1002/(SICI)1522-2683(19990101)20:1<3::AID-ELPS3>3.0.CO;2-U