Diagnostic relevance of chromosomal in-situ hybridization in Merkel cell carcinoma: targeted interphase cytogenetic tumour analyses

• Published in: Histopathology 1999-02, Vol.34 (2), p.163-169
• Main Authors: AMO-TAKYI, K, TIETZE, L, TORY, K, GUERREIRO, P, GÜNTHER, K, BHARDWAJ, R. S, MITTERMAYER, C, HANDT, S
• Format: Article
• Language: English
• Subjects: Adult; Antibodies; Biological and medical sciences; Carcinoma, Merkel Cell - genetics; Carcinoma, Merkel Cell - pathology; Chromosomes, Human; Dermatology; Genes, p53 - genetics; Humans; Immunohistochemistry; In Situ Hybridization; Interphase; interphase cytogenetics; Male; Medical sciences; Merkel cell carcinoma; mutation analysis; Scalp; Sex Chromosomes; Skin Neoplasms - genetics; Skin Neoplasms - pathology; small-cell lung carcinoma; Tumors of the skin and soft tissue. Premalignant lesions
• ISSN: 0309-0167; 1365-2559
• DOI: 10.1046/j.1365-2559.1999.00580.x

Aims To resolve the conflicting diagnoses of five pathologists (which included well‐differentiated neuroendocrine carcinoma, malignant carcinoid, undifferentiated small‐cell carcinoma, primitive neuroectodermal tumour, metastases of small‐cell lung carcinoma (SCLC) and Merkel cell carcinoma (MCC)), and tumour‐free lungs after necropsy, we investigated an alarmingly metastasizing MCC in a 32‐year‐old Caucasian man using chromosomal in‐situ hybridization (CISH). Differences in incidence and course in males and females also prompted targeted analyses for chromosomes X and Y. The lesion was also analysed for p53 gene mutations. Methods and results Paraffin sections of the thorax, buccal lymph nodes and scalp tumours were stained with haematoxylin and eosin. Immunohistochemistry was performed with antibodies against pancytokeratin, keratin 20, neuron‐specific enolase (NSE), chromogranin, neurofilaments and vimentin, among others. Sections (5–6 μm) of the tumours were analysed with α‐satellite probes for chromosomes 1, 6, 7, 11, 12, 17, 18, X and Y using CISH; and exons 5–9 of the p53 gene were examined by polymerase chain reaction and single strand conformation polymorphism (PCR‐SSCP) methods. Although positive for pancytokeratin, keratin 20, chromogranin, NSE, synaptophysin and vimentin, the similarity in antigen profiles expressed by SCLC and MCC prevented a definitive tumour diagnosis. Chromosomal in‐situ hybridization, however, revealed trisomies 1 and 11, two frequent aberrations in MCC, and trisomy 18. Moreover, 71% of the tumour cells had two to three copies of X, whereas 98% of the cell nuclei in the hair follicles and normal epidermis (purported Merkel cell origins) displayed one X chromosome. No mutations were detected in the five exons of the p53 gene examined. Conclusions Had CISH been performed earlier, treatment may have been tailored specifically to suit MCC, since MCC and SCLC have different therapeutic strategies. Finally, chromosome X may be of prognostic relevance in MCC, which apparently predominates in females and yet shows poorer prognosis in males, and hence be worthy of further investigation.