Fingerprinting of mixed bacterial strains and BIOLOG gram-negative (GN) substrate communities by enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR)

PCR-based genomic fingerprinting by use of enterobacterial repetitive intergenic consensus primers (ERIC-PCR) was evaluated for its use in fingerprinting DNA of mixed Gram-negative bacterial strains and BIOLOG Gram-negative (GN) microplate substrate communities. ERIC-PCR fingerprints of six differen...

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Bibliographic Details
Published in:Current microbiology 1999-04, Vol.38 (4), p.217-223
Main Authors: DI GIOVANNI, G. D, WATRUD, L. S, SEIDLER, R. J, WIDMER, F
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Deoxyribonucleic acid
DNA
DNA Fingerprinting - methods
DNA, Bacterial - analysis
Fundamental and applied biological sciences. Psychology
General aspects
Gram-Negative Bacteria - genetics
Gram-Negative Bacteria - growth & development
Gram-Negative Bacteria - isolation & purification
Microbial ecology
Microbiology
Polymerase Chain Reaction - methods
Rhizosphere
Solanum tuberosum - microbiology
Species Specificity
Substrates
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Summary:PCR-based genomic fingerprinting by use of enterobacterial repetitive intergenic consensus primers (ERIC-PCR) was evaluated for its use in fingerprinting DNA of mixed Gram-negative bacterial strains and BIOLOG Gram-negative (GN) microplate substrate communities. ERIC-PCR fingerprints of six different pure bacterial strains and a combined mixture of the strains were compared with fingerprints obtained by two more established methods: amplified ribosomal DNA restriction analysis (ARDRA) and random amplified polymorphic DNA analysis (RAPD-PCR). The ERIC-PCR fingerprint of the mixed strains was highly reproducible and was more species-specific and representative of the individual strain fingerprints than the ARDRA and RAPD-PCR fingerprints, respectively. ERIC-PCR fingerprinting of model and rhizosphere BIOLOG GN substrate communities also provided clearly distinguishable fingerprints. Results of this study suggest that ERIC-PCR represents a rapid and highly discriminating method for fingerprinting DNA of mixed Gram-negative bacterial strains and BIOLOG GN substrate communities.
ISSN:0343-8651
1432-0991
DOI:10.1007/PL00006790