Hydroxyethyl starch reduces the chemotaxis of white cells through endothelial cell monolayers

BACKGROUND: Polymorphonuclear leukocytes (PMNs) play a tremendous role during inflammatory processes. PMNs have to pass a monolayer of endothelial cells to migrate into the extravascular space. Hydroxyethyl starch (HES) is frequently used as a volume expander in critically ill patients. STUDY DESIGN...

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Bibliographic Details
Published in:Transfusion (Philadelphia, Pa.) Pa.), 1999-03, Vol.39 (3), p.289-294
Main Authors: Hofbauer, Roland, Moser, Doris, Hornykewycz, Stephan, Frass, Michael, Kapiotis, Stylianos
Format: Article
Language:English
Subjects:
Adult
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Biological and medical sciences
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
Cells, Cultured
Chemotaxis, Leukocyte - drug effects
Depression, Chemical
Disease Susceptibility
Endothelium, Vascular - cytology
Humans
Hydroxyethyl Starch Derivatives - adverse effects
Hydroxyethyl Starch Derivatives - pharmacology
Infection
Medical sciences
Neutrophils - drug effects
Neutrophils - physiology
Plasma Substitutes - adverse effects
Plasma Substitutes - pharmacology
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
Umbilical Veins
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Summary:BACKGROUND: Polymorphonuclear leukocytes (PMNs) play a tremendous role during inflammatory processes. PMNs have to pass a monolayer of endothelial cells to migrate into the extravascular space. Hydroxyethyl starch (HES) is frequently used as a volume expander in critically ill patients. STUDY DESIGN AND METHODS: The aim of this study was to investigate whether HES influences the chemotaxis of PMNs through endothelial cell monolayers by using a test system that allows the simultaneous treatment of both cell types. Human umbilical endothelial cells were cultured on microporous membrane filters. PMNs were isolated and PMN chemotaxis was studied. RESULTS: The number of untreated PMNs that migrated through untreated endothelial cell monolayers in response to a chemoattractant was used as a control and set as 100 percent. In clinically relevant concentrations, HES was able to significantly decrease PMN chemotaxis through endothelial cell monolayers, showing a dose‐dependent effect (0.1 mg/mL: 99.6 ± 10.9%, p = NS compared to control; 1 mg/mL: 82.4 ± 8.3%, p
ISSN:0041-1132
1537-2995
DOI:10.1046/j.1537-2995.1999.39399219286.x