Prevalence of human gammaretrovirus XMRV in sporadic prostate cancer

Abstract Background We previously identified a novel exogenous gammaretrovirus (xenotropic murine leukemia virus-related gammaretrovirus (XMRV)) using a pan-viral microarray. XMRV is the first MLV-related virus found in human infection. Forty percent (8/20) of familial prostate cancer patients homoz...

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Bibliographic Details
Published in:Journal of clinical virology 2008-11, Vol.43 (3), p.277-283
Main Authors: Fischer, Nicole, Hellwinkel, Olaf, Schulz, Claudia, Chun, Felix K.H, Huland, Hartwig, Aepfelbacher, Martin, Schlomm, Thorsten
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Allergy and Immunology
Biological and medical sciences
Endoribonucleases - genetics
Epidemiology
Fundamental and applied biological sciences. Psychology
Gammaretrovirus - isolation & purification
Germany - epidemiology
Human viral diseases
Humans
Infection
Infectious Disease
Infectious diseases
Male
Medical sciences
Microbiology
Middle Aged
Miscellaneous
Non-familial prostate cancer
Polymerase Chain Reaction - methods
Polymorphism, Genetic
Prevalence
Prostatic Neoplasms - virology
Retroviridae Infections - epidemiology
Reverse Transcriptase Polymerase Chain Reaction - methods
RNase L
RT-PCR
Tumor Virus Infections - epidemiology
Tumors
Viral diseases
Virology
XMRV
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Summary:Abstract Background We previously identified a novel exogenous gammaretrovirus (xenotropic murine leukemia virus-related gammaretrovirus (XMRV)) using a pan-viral microarray. XMRV is the first MLV-related virus found in human infection. Forty percent (8/20) of familial prostate cancer patients homozygous for a mutation in RNase L (R462Q) were positive for XMRV, while the virus was rarely (1/66) detected in familial prostate cancer patients heterozygous for R462Q or carrying the wild type allele. Objectives To determine the presence of XMRV in non-familial prostate cancer samples. Study design RNA from prostate tissue was analyzed for XMRV using nested RT-PCR. In all samples, RNase L (R462Q) genotyping was performed using an allele-specific PCR. Results XMRV-specific sequences were detected in one of 105 tissue samples from non-familial prostate cancer patients and from one of 70 tissue samples from men without prostate cancer. The two XMRV-positive patients were wild type or heterozygous for the R462Q mutation and thus carried at least one fully functional RNase L allele. Conclusions XMRV was rarely detected in non-familial prostate cancer samples from Northern European patients. The homozygous mutation R462Q (QQ) was significantly underrepresented (
ISSN:1386-6532
1873-5967
DOI:10.1016/j.jcv.2008.04.016