Purification of a mannose/glucose-specific hemagglutinin/lectin from a Vibrio cholerae O1 strain

Abstract A cell-associated mannose/glucose-specific hemagglutinin (MSHA) has been purified from a strain of Vibrio cholerae O1 by chromatography on a chitin column followed by affinity purification on Sephadex G100. The purified protein gave a single stained band of 40 kDa by SDS-PAGE, exhibited hig...

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Bibliographic Details
Published in:FEMS immunology and medical microbiology 1999-03, Vol.23 (3), p.221-227
Main Authors: Sasmal, D., Guhathakurta, B., Ghosh, A.N., Pal, C.R., Datta, A.
Format: Article
Language:English
Subjects:
Affinity
Animals
Antisera
Bacteria
Bacteriology
Biological and medical sciences
Chitin
Cholera
Column chromatography
Fucose
Fundamental and applied biological sciences. Psychology
Gel electrophoresis
Glucosamine
Glucose
Hemagglutinin
Hemagglutinins
Hemagglutinins - immunology
Hemagglutinins - isolation & purification
Homology
Immunodiffusion
Labeling
Lectin
Lectins - immunology
Lectins - isolation & purification
Mannose
Microbiology
N-Acetylglucosamine
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Protein purification
Proteins
Purification
Rabbits
Sodium lauryl sulfate
Vibrio cholerae
Vibrio cholerae - chemistry
Vibrio cholerae O1
Waterborne diseases
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Summary:Abstract A cell-associated mannose/glucose-specific hemagglutinin (MSHA) has been purified from a strain of Vibrio cholerae O1 by chromatography on a chitin column followed by affinity purification on Sephadex G100. The purified protein gave a single stained band of 40 kDa by SDS-PAGE, exhibited high affinity towards d-mannose and d-glucose but was resistant to l-fucose and N-acetyl-d-glucosamine. The purified MSHA was revealed as a globular form of protein under electron microscope. In immunodiffusion tests the purified MSHA produced a single precipitin band against homologous antisera and antisera raised against the whole cell bacteria without any reactivity towards antisera raised against the purified N-acetyl-d-glucosamine-specific lectin of the same bacterial strain. Immunogold labelling confirmed the location of hemagglutinin on the surface of the bacteria. Purified MSHA reacted strongly with sera from convalescent cholera patients in immunodiffusion tests.
ISSN:0928-8244
1574-695X
2049-632X
DOI:10.1111/j.1574-695X.1999.tb01242.x