Metabolism in single rat brain slices measured by magnetic resonance spectroscopy

Nuclear magnetic resonance spectroscopy (MRS) has been used to study brain biochemistry in superfused brain slice preparations for over a decade. However, unlike techniques that monitor electrical activity, ion fluxes, or the release of radio-labeled compounds in single brain slices, MRS studies hav...

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Bibliographic Details
Published in:Brain research. Brain research protocols 1999-04, Vol.4 (1), p.97-102
Main Authors: Gasparovic, Charles, King, Delaine, Feeney, Dennis M
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Anaerobiosis - physiology
Animals
Brain - metabolism
Brain slice
Energy Metabolism - physiology
Hippocampus - metabolism
In Vitro Techniques
Lactic Acid - metabolism
Magnetic Resonance Spectroscopy - methods
Neocortex - metabolism
Nuclear magnetic resonance
Phosphocreatine - metabolism
Prosencephalon - metabolism
Rats
Rats, Sprague-Dawley
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Summary:Nuclear magnetic resonance spectroscopy (MRS) has been used to study brain biochemistry in superfused brain slice preparations for over a decade. However, unlike techniques that monitor electrical activity, ion fluxes, or the release of radio-labeled compounds in single brain slices, MRS studies have required samples composed of several slices and inherently poor anatomical specificity in order to achieve adequate signal-to-noise levels, spectral resolution, or, in the case of 1 H MRS, a high degree of artifact-free water signal suppression. We report that gradient-enhanced 1 H MRS techniques combined with a simple slice positioning and perfusion technique yield high-quality spectra from single 400 μm rat forebrain or neocortical–hippocampal slices within 15 min of data acquisition time. Spectra of comparable quality were obtained from samples with three neocortical or three hippocampal slices within the same time frame. The assessment of anaerobic energy metabolism in single slices by 1 H MRS is also demonstrated. In addition to greater anatomical resolution in studies on brain slice biochemistry, single slice MRS also presents the possibility of correlating, within the same slice, 1 H MRS-detectable metabolite levels with other physiological measurements commonly performed on single brain slices. Themes: Cellular and Molecular Biology Topics: Staining, tracing, and imaging techniques
ISSN:1385-299X
DOI:10.1016/S1385-299X(99)00010-0