Cytogenetic alignment of the bovine chromosome 13 genome map by fluorescence in-situ hybridization of human chromosome 10 and 20 comparative markers

A bovine bacterial artificial chromosome (BAC) library was screened for the presence of six genes (IL2RA, VIM, THBD, PLC-II, CSNK2A1 and TOP1) previously assigned to human chromosomes 10 or 20 (HSA10 or HSA20). Four of the genes were found represented in the bovine BAC library by at least one clone....

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Bibliographic Details
Published in:Chromosome research 1999-01, Vol.7 (2), p.115-119
Main Authors: Gallagher, D.S. Jr, Schlapfer, J, Burzlaff, J.D, Womack, J.E, Stelly, D.M, Davis, S.K, Taylor, J.F
Format: Article
Language:English
Subjects:
Animals
Artificial chromosomes
Casein Kinase II
Cattle
Chromosome Mapping
chromosomes
Chromosomes, Human, Pair 10
Chromosomes, Human, Pair 20
Genetic Markers
Genetics
Humans
In Situ Hybridization, Fluorescence - methods
nucleic acid hybridization
Phosphatidylinositol Diacylglycerol-Lyase
Protein-Serine-Threonine Kinases - genetics
Receptors, Interleukin-2 - genetics
Thrombomodulin - genetics
Type C Phospholipases - genetics
Vimentin - genetics
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Summary:A bovine bacterial artificial chromosome (BAC) library was screened for the presence of six genes (IL2RA, VIM, THBD, PLC-II, CSNK2A1 and TOP1) previously assigned to human chromosomes 10 or 20 (HSA10 or HSA20). Four of the genes were found represented in the bovine BAC library by at least one clone. The identified BAC clones were used as probes in single-color fluorescence in-situ hybridization (FISH) to determine the chromosomal band location of each gene. As predicted by the human/bovine comparative map and comparative chromosome painting analysis, the four genes mapped to bovine chromosome 13 (BTA13). Dual-color FISH was then used to integrate these four type I markers into the existing BTA13 genome map. These FISH results anchor the BTA13 genome map from bands 14-23, and confirm the presence of a conserved HSA10 homologous synteny group on BTA13 centromeric to a HSA20 homologous segment.
ISSN:0967-3849
1573-6849
DOI:10.1023/A:1009243015544