Fas-Activated Apoptosis and Apoptosis Mediators in Human Trabecular Meshwork Cells

A gradual loss of cells occurs within the human trabecular meshwork during normal aging and appears to be increased in patients with primary open-angle glaucoma. The exact mechanism by which cells are lost in either condition is not known, however phagocytosis, cell migration and cell death have bee...

Full description

Saved in:
Bibliographic Details
Published in:Experimental eye research 1999-05, Vol.68 (5), p.583-590
Main Authors: AGARWAL, RAJNEE, TALATI, MIHIR, LAMBERT, WENDI, CLARK, ABBOT F, WILSON, STEVEN E, AGARWAL, NEERAJ, WORDINGER, ROBERT J
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Aging - physiology
Antibodies, Monoclonal - pharmacology
Apoptosis
Bax
Bcl-2
bcl-2-Associated X Protein
bcl-X Protein
Bcl-xl
Biological and medical sciences
Cell Line
Child, Preschool
Cysteine Proteinase Inhibitors - genetics
DNA Fragmentation
Fas
Fas ligand
Fas Ligand Protein
fas Receptor - genetics
fas Receptor - immunology
fas Receptor - metabolism
glaucoma
Glaucoma and intraocular pressure
Humans
ICE
In Situ Nick-End Labeling
Infant
Infant, Newborn
Medical sciences
Membrane Glycoproteins - metabolism
Middle Aged
Ophthalmology
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-bcl-2 - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Serpins - genetics
trabecular meshwork
Trabecular Meshwork - cytology
Trabecular Meshwork - physiology
Viral Proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A gradual loss of cells occurs within the human trabecular meshwork during normal aging and appears to be increased in patients with primary open-angle glaucoma. The exact mechanism by which cells are lost in either condition is not known, however phagocytosis, cell migration and cell death have been suggested. Apoptosis is one method by which cell death can occur. We have examined the modulators for apoptosis within the human trabecular meshwork using both cell lines and ex-vivo dissected trabecular meshwork tissues obtained from normal donors. Using RT-PCR it was shown that mRNA for several modulators of apoptosis (Fas, Bcl-2, Bcl-xl, Bax, and ICE) are expressed by both cell lines and ex-vivo tissues. Apoptosis was stimulated to occur by treating cell lines with a monoclonal antibody (IgM) to Fas. Apoptosis was verified via morphological changes to the cells, transferase-mediated dUTP nick-end labeling TUNEL Immunofluorescence, and DNA laddering. Control cells exposed to IgM did not undergo apoptosis. These results represent the first report of apoptosis modulators within the human trabecular meshwork and demonstrate that human trabecular meshwork cells can be stimulated to undergo apoptosis via the Fas/FasL pathway.
ISSN:0014-4835
1096-0007
DOI:10.1006/exer.1998.0636